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Anti-IRS1, agarose conjugate

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货号：16-146

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
A IP Rb Gel Immobilized Polyclonal Antibody
Description
Catalogue Number 16-146
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-IRS1 Antibody, agarose conjugate
Overview Anti-IRS-1 (06-248) covalently coupled to protein A agarose using dimethylpimelimidate.
Alternate Names
  • insulin receptor substrate 1
Background Information Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
References
Product Information
Format Gel Immobilized
Control
  • 3T3/A31 mouse fibroblasts cell lysate.
Presentation Gel immobilized rabbit polyclonal IgG bound to beads; provided as a 50% gel slurry in PBS containing 0.05% azide. Liquid suspension.
Applications
Application This Anti-IRS1 Antibody, agarose conjugate is validated for use in IP for the detection of IRS1.
Key Applications
  • Immunoprecipitation
Application Notes Affinity Purification:
The antibody-agarose may be useful for affinity purification of proteins that bind IRS1.

Suitable for immunoprecipitation and affinity purification.

Elution Buffer (IRS1 Released)
SDS-Page sample buffer (100%)
0.5M Glycine, pH 2.0 (0%)
0.1M TEA, pH 11.5 (0%)
Biological Information
Immunogen Carboxy-terminal 14 amino acid peptide of rat liver IRS1 (C-YASINFQKQPEDRQ).
Epitope C-terminal of IRS1
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes IRS1, Mr 160-185kDa.
Isotype IgG
Species Reactivity
  • All
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIRS-1
  • IRS-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
SIZE: 1242 amino acids; 131591 Da
SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).
DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Molecular Weight 165 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoprecipitating IRS-1 from a RIPA lysate of 3T3/A31 mouse fibroblasts.

Immunoprecipitation: 10-25 μg (20-50 μL of 50% slurry) of this lot was used to immunoprecipitate IRS1 from 500 μg of a RIPA lysate of 3T3/A31 mouse fibroblasts. After boiling in SDS-PAGE sample buffer, a small amount of the non-covalently bound IgG light chain (28 kDa) and heavy chain (55 kDa) may be observed.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 6 months at 2-8ºC from date of receipt.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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