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Anti-Transcription Factor PU.1, clone EPR3159Y, Rabbit Monoclonal

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号:04-1072

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, FC, ELISA M Ascites Monoclonal Antibody
Description
Catalogue Number MABE632
Replaces 04-1072
Description Anti-SPI1, clone 5C7 Antibody
Alternate Names
  • Transcription factor PU.1
  • 31 kDa-transforming protein
  • SPI1
Background Information Transcription factor PU.1 (SPI1) is a transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes.
References
Product Information
Format Ascites
Presentation Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Applications
Application Anti-SPI1, clone 5C7 Antibody is an antibody against SPI1 for use in Western Blotting, Flow Cytometry, ELISA.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • ELISA
Application Notes Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected SPI1 in HeLa cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Biological Information
Immunogen Purified recombinant fragment of human SPI1 expressed in E. Coli. 
Clone 5C7
Concentration Please refer to lot specific datasheet.
Host Mouse
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Gene Symbol
  • SPI1
Purification Method Unpurified
UniProt Number
Molecular Weight ~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in SPI1 recombinant protein, HEK293 and hIgGFc transfected HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected SPI1 in SPI1 recombinant protein and hIgGFc transfected HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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