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Anti-BrdU, clone BU-1 Alexa Fluor® 488 conjugated

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货号：FCMAB101A4

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC, ICC M AlexaFluor®488 Monoclonal Antibody
Description
Catalogue Number FCMAB101A4
Trade Name
  • Milli-Mark
Description Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated
Alternate Names
  • BrdU
Background Information Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
References
Product Information
Format AlexaFluor®488
Control
  • After incorporation of BrdU, all DNA containing species
Presentation Presentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Applications
Application Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Biological Information
Immunogen Bromodeoxyuridine
Clone Bu-1
Host Mouse
Specificity Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
Isotype IgG2a
Species Reactivity
  • Human
Species Reactivity Note Tested on Human. Predicted to react with all species.
Antibody Type Monoclonal Antibody
Gene Symbol
  • BrdU
Purification Method Protein G Purified
Molecular Weight none given
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by flow cytometry with Jurkat Cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 6 months at 2-8°C from date of receipt. Protect from light.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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