Anti-RAR?, clone 9?-9A6 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ICC

Ascites

Monoclonal Antibody

Description
Catalogue Number	04-1545
Description	Anti-RARα Antibody, clone 9α-9A6
Alternate Names	Nuclear receptor subfamily 1 group B member 1 Retinoic acid receptor, alpha polypeptide nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form retinoic acid receptor, alpha
Background Information	Retinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ.

Description

Catalogue Number

04-1545

Description

Anti-RARα Antibody, clone 9α-9A6

Alternate Names

Nuclear receptor subfamily 1 group B member 1
Retinoic acid receptor, alpha polypeptide
nucleophosmin-retinoic acid receptor alpha fusion protein
NPM-RAR long form
retinoic acid receptor, alpha

Background Information

Retinoic acid receptor alpha (RARa) is a nuclear receptor encoded by the RARA gene. This gene is sometimes referred to as NR1B1 (nuclear receptor subfamily 1, group B, member 1). RARa is a member of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. As implied by its name, this protein is a receptor for retinoic acid, a metabolite of vitamin A. Retinoic acid is necessary for normal organogenesis, but can act as a teratogen when present at high levels during embryonic and fetal development. During fetal and embryonic development retinoic acid regulates transcription through the activation of nuclear receptors, specifically the Retinoic Acid Receptor (RAR). Signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR), which form RXR/RAR heterodimers. There are three isoforms associated with RAR; RARα, RARβ and RARγ.

Product Information
Format	Ascites
Control	HL60 cell lysate
Presentation	Unpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Product Information

Format

Ascites

Control

HL60 cell lysate

Presentation

Unpurified mouse monoclonal IgG1κ ascites with 0.05% sodium azide.

Applications
Application	Anti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RARα in HeLa and A431 cells.

Applications

Application

Anti-RARα Antibody, clone 9α-9A6 is a Mouse Monoclonal Antibody for detection of RARα also known as Retinoic acid receptor alpha polypeptide & has been validated in WB, ICC.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected RARα in HeLa and A431 cells.

Biological Information
Immunogen	Recombinant protein corresponding to human RARα.
Epitope	Unknown
Clone	clone 9α-9A6
Host	Mouse
Specificity	This antibody recognizes RARα.
Isotype	IgG1κ
Species Reactivity	Human
Species Reactivity Note	Demonstrated to react with human. Homology to mouse and rat is 91%.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000955.1
Entrez Gene Summary	Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 7468032]).[supplied by OMIM].
Gene Symbol	NR1B1 RAR RARA RAR-alpha
Purification Method	Unpurified
UniProt Number	P10276
UniProt Summary	FUNCTION: This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression. SIZE: 462 amino acids; 50771 Da SUBUNIT: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand- inducible manner. SUBCELLULAR LOCATION: Nucleus. DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). DISEASE: Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM. SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
Molecular Weight	58 kDa observed

Biological Information

Immunogen

Recombinant protein corresponding to human RARα.

Epitope

Unknown

Clone

clone 9α-9A6

Host

Mouse

Specificity

This antibody recognizes RARα.

Isotype

IgG1κ

Species Reactivity

Human

Species Reactivity Note

Demonstrated to react with human. Homology to mouse and rat is 91%.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_000955.1

Entrez Gene Summary

Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 7468032]).[supplied by OMIM].

Gene Symbol

NR1B1
RAR
RARA
RAR-alpha

Purification Method

Unpurified

UniProt Number

P10276

UniProt Summary

FUNCTION: This is a receptor for retinoic acid. This metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and is a powerful teratogen. This receptor controls cell function by directly regulating gene expression.

SIZE: 462 amino acids; 50771 Da

SUBUNIT: Interacts with CDK7 (By similarity). Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NOCA7 in a ligand- inducible manner.

SUBCELLULAR LOCATION: Nucleus.

DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.

PTM: Phosphorylated. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity).

DISEASE: Chromosomal aberrations involving RARA may be a cause of acute promyelocytic leukemia (APL). Translocation t(11;17)(q32;q21) with ZBTB16/PLZF; translocation t(15;17)(q21;q21) with PML; translocation t(5;17)(q32;q11) with NPM.

SIMILARITY: Belongs to the nuclear hormone receptor family. NR1 subfamily. & Contains 1 nuclear receptor DNA-binding domain.

Molecular Weight

58 kDa observed

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HL60 cell lysate. Western Blot Analysis: 1:1,000 dilution of this antibody detected RARα on 10 µg of HL60 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HL60 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected RARα on 10 µg of HL60 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-RAR?, clone 9?-9A6 (mouse monoclonal)

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