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Anti-Germin2, clone 2E17 (mouse monoclonal)

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货号：05-1540

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC, ELISA, IP M Purified Monoclonal Antibody
Description
Catalogue Number 05-1540
Description Anti-Gemin2 Antibody, clone 2E17
Alternate Names
  • GEMIN2
  • Gemin2
  • Component of gems
  • SMN interacting protein 1-delta
  • SMN-interacting protein 1
  • survival interacting protein 1
  • survival of motor neuron protein interacting protein
Background Information Gemin-2 (also known as SIP1 or SMN interacting protein1) is a part of the SMN complex. The SMN complex plays a role in RNA metabolism and in splicing regulation. As part of the SMN complex Gemin-2 interacts directly with Gemin-5 in a heteromeric complex that includes Gemin-3, Gemin-4, Gemin-6. This macromolecular complex (SMN complex) mediates assembly of spliceosomal snRNA complexes containing U1 and U5.
References
Product Information
Format Purified
Control
  • HeLa cell lysates.
Presentation Purified mouse IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Applications
Application Anti-Gemin2 Antibody, clone 2E17 is a Mouse Monoclonal Antibody for detection of Gemin2 also known as SMN interacting protein 1-delta & has been validated in WB, ICC, ELISA & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • ELISA
  • Immunoprecipitation
Application Notes Immunocytochemistry:
Cited in Battle, D.J., et. al (2007). J. Biol. Chem. 282: 27953 - 27959 and Zhang, Z., et. al (2008). Cell, 133:85-600. using a representative lot.
ELISA:
Cited in Zhang, Z., et. al (2008). Cell, 133:85-600.
Immunoprecipitation:
Cited in Battle, D.J., et. al(2007). J. Biol. Chem. 282: 27953 - 27959 using a representative lot.
Biological Information
Immunogen His-tagged recombinant protein corresponding to human Gemin-2.
Epitope N/A
Clone 2E17
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Antibody recognizes Gemin-2.
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Reacts with Human. Predicted to recognize mouse based on 100% sequence homology.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SIP1
  • SIP1-delta
Purification Method Protein G Purified
UniProt Number
UniProt Summary Function The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus.

Subunit structure Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN5.
Molecular Weight 32 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in HeLa cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Gemin-2 in HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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