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Anti-phospho-eNOS/NOS III (Ser617)

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货号：07-561

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
B WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number 07-561
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-eNOS/NOS III (Ser617) Antibody
Overview Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
References
Product Information
Format Affinity Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Anti-phospho-eNOS/NOS III (Ser617) Antibody detects level of phospho-eNOS/NOS III (Ser617) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen Peptide corresponding to amino acids surrounding phosphorylated Ser617 of eNOS/NOS III. This site corresponds to human Ser615.
Host Rabbit
Specificity phosphorylated eNOS/NOS III (Ser617)
Isotype IgG
Species Reactivity
  • Bovine
Species Reactivity Note The immunizing sequence has 11/12 identical amino acids in mouse, and canine.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
  • cNOS
  • ECNOS
  • eNOS
  • EC-NOS
  • NOSIII
Modifications
  • Phosphorylation
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Molecular Weight 132kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot in RIPA lysates from fetal bovine heart endothelial (FBHE) cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -20°C
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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