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Anti-HDAC1, clone 2E10 (Ms Monoclonal IgG1, culture supernatant)

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货号：05-100

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP M Purified Monoclonal Antibody
Description
Catalogue Number 05-100-I
Replaces 05-100
Brand Family Upstate
Description Anti-HDAC1 Antibody, clone 2E10
Alternate Names
  • HDAC1
  • Histone deacetylase 1
  • HD1
Background Information Histone deacetylase 1 is a nuclear enzyme encoded by the Hdac1 gene (also known as HD1, RPD3, MommeD5, Hdac1-ps) in mouse and HDAC1 (also known as GoON-10, RPD3L1) gene in human. Hdac1 plays an important role in epigenetic regulation of gene expression by catalyzing the hydrolysis of N(6)-acetylated histone lysine residues. Hdac1 is also known to deacetylate p53 and thereby downregulate p53 activity.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-HDAC1 Antibody, clone 2E10 is an antibody against HDAC1 for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Immunoprecipitation Analysis A representative lot was used to immunoprecipitate HDAC1 from HeLa cell lysate, followed by immune complex enzyme activity assay using HDAC Assay Kit (Fluorometric Detection), Cat. No. 17-356.
Biological Information
Immunogen KLH-conjugated, synthetic peptide (C-EEKPEAKGVKEEVKLA) corresponding to amino acids 467-482 of mouse HDAC1. The immunizing sequence is identical in human.
Clone 2E10
Concentration Please refer to lot specific datasheet.
Host Mouse
Isotype IgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Hdac1
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~55 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HDAC1 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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