Anti-HDAC2, clone Y461 (rabbit monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, B, M, R, Ht	ICC, IP, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, B, M, R, Ht

ICC, IP, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-814
Replaces	04-229
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-HDAC2 Antibody, clone 3F3
Alternate Names	YY1-associated factor 1 histone deacetylase 2 transcriptional regulator homolog RPD3
Background Information	Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Description

Catalogue Number

05-814

Replaces

04-229

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-HDAC2 Antibody, clone 3F3

Alternate Names

YY1-associated factor 1
histone deacetylase 2
transcriptional regulator homolog RPD3

Background Information

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Product Information
Format	Purified
Control	Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Presentation	Purified mouse monoclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Product Information

Format

Purified

Control

Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Presentation

Purified mouse monoclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Applications
Application	Anti-HDAC2 Antibody, clone 3F3 is a Mouse Monoclonal Antibody for detection of HDAC2 also known as YY1-associated factor 1, histone deacetylase 2 & has been validated in ICC, IP & WB.
Key Applications	Immunocytochemistry Immunoprecipitation Western Blotting
Application Notes	Immunocytochemistry: This antibody has been reported by an independent laboratory to show positive immunostaining for HDAC2 in NIH/3T3 and U2OS cells fixed with paraformaldehyde. Immunoprecipitation and HDAC Assay: 3 μL of a previous lot was used to immunoprecipitate HDAC activity from HeLa nuclear extract, which was then measured using the HDAC Assay Kit (Fluorometric Detection) (Catalog # 17-356). An independent laboratory has also demonstrated positive immunoprecipitation /HDAC assay results using NIH/3T3 cells.

Applications

Application

Anti-HDAC2 Antibody, clone 3F3 is a Mouse Monoclonal Antibody for detection of HDAC2 also known as YY1-associated factor 1, histone deacetylase 2 & has been validated in ICC, IP & WB.

Key Applications

Immunocytochemistry
Immunoprecipitation
Western Blotting

Application Notes

Immunocytochemistry:
This antibody has been reported by an independent laboratory to show positive immunostaining for HDAC2 in NIH/3T3 and U2OS cells fixed with paraformaldehyde.

Immunoprecipitation and HDAC Assay:
3 μL of a previous lot was used to immunoprecipitate HDAC activity from HeLa nuclear extract, which was then measured using the HDAC Assay Kit (Fluorometric Detection) (Catalog # 17-356). An independent laboratory has also demonstrated positive immunoprecipitation /HDAC assay results using NIH/3T3 cells.

Biological Information
Immunogen	KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to amino acids 473-488 of human HDAC2 (Accession # NP_001518) containing a N-terminal cysteine
Clone	3F3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes HDAC2, MW ~60 kDa.
Isotype	IgG1
Species Reactivity	Human Bovine Mouse Rat Hamster
Species Reactivity Note	Human, mouse, bovine and hamster. Predicted to cross-react with rat based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001518
Entrez Gene Summary	This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Gene Symbol	HDAC2 HD2 YAF1 RPD3
Purification Method	Protein G Purified
UniProt Number	Q92769
UniProt Summary	FUNCTION: SwissProt: Q92769 # Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. SIZE: 488 amino acids; 55364 Da SUBUNIT: Interacts with the non-histone region of H2AFY (By similarity). Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Part of a complex containing the core histones H2A, H2B, H3 and H4, DEK and unphosphorylated DAXX. Part of a complex containing ATR and CHD4. Forms a heterologous complex at least with YY1. Interacts with ATR, DNMT1, MINT, HDAC7, HDAC10, HCFC1, NRIP1, MJD2A/JHDM3A, SAP30, SETDB1 and SUV39H1. Interacts with the non-histone region of H2AFY. Interacts with PELP1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with CBFA2T3. Interacts with SAP30L. SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Widely expressed; lower levels in brain and lung. SIMILARITY: SwissProt: Q92769 ## Belongs to the histone deacetylase family. Type 1 subfamily.
Molecular Weight	~60 kDa

Biological Information

Immunogen

KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to amino acids 473-488 of human HDAC2 (Accession # NP_001518) containing a N-terminal cysteine

Clone

3F3

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes HDAC2, MW ~60 kDa.

Isotype

IgG1

Species Reactivity

Human
Bovine
Mouse
Rat
Hamster

Species Reactivity Note

Human, mouse, bovine and hamster. Predicted to cross-react with rat based on sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_001518

Entrez Gene Summary

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Gene Symbol

HDAC2
HD2
YAF1
RPD3

Purification Method

Protein G Purified

UniProt Number

Q92769

UniProt Summary

FUNCTION: SwissProt: Q92769 # Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1.
SIZE: 488 amino acids; 55364 Da
SUBUNIT: Interacts with the non-histone region of H2AFY (By similarity). Part of the core histone deacetylase (HDAC) complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core complex associates with MTA2, MBD3, MTA1L1, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylation (NuRD) complex, or with SIN3, SAP18 and SAP30 to form the SIN3 HDAC complex. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Part of a complex containing the core histones H2A, H2B, H3 and H4, DEK and unphosphorylated DAXX. Part of a complex containing ATR and CHD4. Forms a heterologous complex at least with YY1. Interacts with ATR, DNMT1, MINT, HDAC7, HDAC10, HCFC1, NRIP1, MJD2A/JHDM3A, SAP30, SETDB1 and SUV39H1. Interacts with the non-histone region of H2AFY. Interacts with PELP1. Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts with CBFA2T3. Interacts with SAP30L.
SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Widely expressed; lower levels in brain and lung.
SIMILARITY: SwissProt: Q92769 ## Belongs to the histone deacetylase family. Type 1 subfamily.

Molecular Weight

~60 kDa

Product Usage Statements
Quality Assurance	Evaluated by western blot on nuclear extracts from HeLa cells. Western Blot Analysis: 0.5-2 μg/mL of this antibody detected HDAC2 in nuclear extracts from HeLa cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by western blot on nuclear extracts from HeLa cells.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected HDAC2 in nuclear extracts from HeLa cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-HDAC2, clone Y461 (rabbit monoclonal)

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