Anti-Ataxin-7, clone 3SCA-1C1 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M, H	WB	M	Ascites	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, M, H

Ascites

Monoclonal Antibody

Description
Catalogue Number	04-1573
Description	Anti-Ataxin-7 Antibody, clone 3SCA-1C1
Alternate Names	ADCAII, OPCA3, SCA7
Background Information	Ataxin-7 is a protein of unknown function. However, since this protein contains a nuclear localization sequence and is found to be localized in the nucleus, it has been postulated to be a potential transcription factor. Spinocerebellar ataxia-7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded CAG repeat in the gene encoding ataxin-7 (the diseased allele associated with Spinocerebellar ataxia-7, contains 38-130 CAG repeats (near the N-terminus), compared to 7-17 in the normal allele). In cells where there is a mutation of the SCA7 gene, ataxin-7 accumulates in intranuclear inclusions and can result in cell death. Spinocerebellar ataxia (SCA) is one of a group of genetic disorders characterized by slowly progressive incoordination of gait and often associated with poor coordination of hands, speech, and eye movements. Frequently, atrophy of the cerebellum occurs.

Description

Catalogue Number

04-1573

Description

Anti-Ataxin-7 Antibody, clone 3SCA-1C1

Alternate Names

ADCAII, OPCA3, SCA7

Background Information

Ataxin-7 is a protein of unknown function. However, since this protein contains a nuclear localization sequence and is found to be localized in the nucleus, it has been postulated to be a potential transcription factor. Spinocerebellar ataxia-7 (one of a group of hereditary neurodegenrative diseases) is caused by an expanded CAG repeat in the gene encoding ataxin-7 (the diseased allele associated with Spinocerebellar ataxia-7, contains 38-130 CAG repeats (near the N-terminus), compared to 7-17 in the normal allele). In cells where there is a mutation of the SCA7 gene, ataxin-7 accumulates in intranuclear inclusions and can result in cell death. Spinocerebellar ataxia (SCA) is one of a group of genetic disorders characterized by slowly progressive incoordination of gait and often associated with poor coordination of hands, speech, and eye movements. Frequently, atrophy of the cerebellum occurs.

Product Information
Format	Ascites
Control	Rat brain tissue lysate.
Presentation	Unpurified mouse IgG1, k antibody in ascites fluid without preservatives.

Product Information

Format

Ascites

Control

Rat brain tissue lysate.

Presentation

Unpurified mouse IgG1, k antibody in ascites fluid without preservatives.

Applications
Application	Anti-Ataxin-7 Antibody, clone 3SCA-1C1 is an antibody against Ataxin-7 for use in WB.
Key Applications	Western Blotting
Application Notes	ELISA: A 1:500-1:5,000 dilution of a previous lot of 04-1573 was used in ELISA, as reported by an outside laboratory. Immunocytochemistry: A 1:500-1:5,000 dilution of a previous lot of 04-1573 was used in IC, as reported by an outside laboratory.

Applications

Application

Anti-Ataxin-7 Antibody, clone 3SCA-1C1 is an antibody against Ataxin-7 for use in WB.

Key Applications

Western Blotting

Application Notes

ELISA: A 1:500-1:5,000 dilution of a previous lot of 04-1573 was used in ELISA, as reported by an outside laboratory. Immunocytochemistry: A 1:500-1:5,000 dilution of a previous lot of 04-1573 was used in IC, as reported by an outside laboratory.

Biological Information
Immunogen	Recombinant human Ataxin 7 as a fusion protein (aa 1 to aa 229).
Epitope	Epitope mapped between aa 66 and aa 119.
Clone	clone 3SCA-1C1
Host	Mouse
Specificity	This antibody recognizes Ataxin-7 protein.
Isotype	IgG1κ
Species Reactivity	Rat Mouse Human
Species Reactivity Note	Demonstrated to react with Rat and Mouse. Predicted to react with Human based on immunogen design.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000324
Entrez Gene Summary	The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. This locus has been mapped to chromosome 3, and it has been determined that the diseased allele associated with Spinocerebellar ataxia-7, contains 38-130 CAG repeats (near the N-terminus), compared to 7-17 in the normal allele. The exact function of this gene is not known, however, since the encoded protein contains a nuclear localization sequence, and is found to be localized in the nucleus, it has been postulated to be a potential transcription factor. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene.
Gene Symbol	ATXN7
Purification Method	Unpurified
UniProt Number	O15265
UniProt Summary	UniProtKB/Swiss-Prot: ATX7_HUMAN, O15265 (See protein sequence) Recommended Name: Ataxin-7 Size: 892 amino acids; 95451 Da Subunit: Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP, TAF9 and ATXN7. The STAGA core complex is associated with a subcomplex required for histone deubiquitinylation composed of ATXN7L3, ENY2 and USP22. Interacts with SORBS1, PSMC1 and CRX. Interacts with TRRAP, GCN5L2 and TAF10 Subcellular location: Nucleus. Nucleus, nucleolus. Nucleus matrix. Note=In addition to a diffuse distribution throughout the nucleus, it is associated with the nuclear matrix and the nucleolus Subcellular location: Isoform b: Cytoplasm Secondary accessions: O75328 O75329 Q9Y6P8 Alternative splicing: 2 isoforms: O15265-1 O15265-2
Molecular Weight	96 kDa Calculated

Biological Information

Immunogen

Recombinant human Ataxin 7 as a fusion protein (aa 1 to aa 229).

Epitope

Epitope mapped between aa 66 and aa 119.

Clone

clone 3SCA-1C1

Host

Mouse

Specificity

This antibody recognizes Ataxin-7 protein.

Isotype

IgG1κ

Species Reactivity

Rat
Mouse
Human

Species Reactivity Note

Demonstrated to react with Rat and Mouse. Predicted to react with Human based on immunogen design.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_000324

Entrez Gene Summary

The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. This locus has been mapped to chromosome 3, and it has been determined that the diseased allele associated with Spinocerebellar ataxia-7, contains 38-130 CAG repeats (near the N-terminus), compared to 7-17 in the normal allele. The exact function of this gene is not known, however, since the encoded protein contains a nuclear localization sequence, and is found to be localized in the nucleus, it has been postulated to be a potential transcription factor. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene.

Gene Symbol

ATXN7

Purification Method

Unpurified

UniProt Number

O15265

UniProt Summary

UniProtKB/Swiss-Prot: ATX7_HUMAN, O15265 (See protein sequence)

Recommended Name: Ataxin-7

Size: 892 amino acids; 95451 Da

Subunit: Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H,

GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP, TAF9 and ATXN7. The STAGA core

complex is associated with a subcomplex required for histone deubiquitinylation composed of

ATXN7L3, ENY2 and USP22. Interacts with SORBS1, PSMC1 and CRX. Interacts with TRRAP, GCN5L2 and

TAF10

Subcellular location: Nucleus. Nucleus, nucleolus. Nucleus matrix. Note=In addition to a diffuse

distribution throughout the nucleus, it is associated with the nuclear matrix and the nucleolus

Subcellular location: Isoform b: Cytoplasm

Secondary accessions: O75328 O75329 Q9Y6P8

Alternative splicing: 2 isoforms: O15265-1 O15265-2

Molecular Weight

96 kDa Calculated

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting of Rat brain lysate. Western Blotting: Representative lot data: A 1:1000 dilution of this antibody detected Ataxin-7 in 10ug of Rat brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting of Rat brain lysate. Western Blotting: Representative lot data: A 1:1000 dilution of this antibody detected Ataxin-7 in 10ug of Rat brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Ataxin-7, clone 3SCA-1C1 (mouse monoclonal)

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