Anti-MAGOH, clone 21B12 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, IP, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1538
Description	Anti-MAGOH Antibody, clone 21B12
Alternate Names	mago-nashi (Drosophila) homolog, proliferation-associated mago-nashi homolog mago-nashi homolog, proliferation-associated (Drosophila)
Background Information	MAGOH is a component of the splicing-dependent multiprotein exon junction complex (EJC) found at splice junctions on mRNAs. The EJC core complex contains CASC3, EIF4A3, MAGOH and Y-14 and assembles approximately 20 nucleotides upstream of exon-exon junctions. This complex functions in post-splicing events, such as nuclear export and nonsense-mediated decay of mRNA. Core components of the EJC remain bound to spliced mRNAs throughout all stages of mRNA metabolism and mark the position of the exon-exon junctions in mature mRNA.

Description

Catalogue Number

05-1538

Description

Anti-MAGOH Antibody, clone 21B12

Alternate Names

mago-nashi (Drosophila) homolog, proliferation-associated
mago-nashi homolog
mago-nashi homolog, proliferation-associated (Drosophila)

Background Information

MAGOH is a component of the splicing-dependent multiprotein exon junction complex (EJC) found at splice junctions on mRNAs. The EJC core complex contains CASC3, EIF4A3, MAGOH and Y-14 and assembles approximately 20 nucleotides upstream of exon-exon junctions. This complex functions in post-splicing events, such as nuclear export and nonsense-mediated decay of mRNA. Core components of the EJC remain bound to spliced mRNAs throughout all stages of mRNA metabolism and mark the position of the exon-exon junctions in mature mRNA.

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Purified

Control

HeLa cell lysate

Presentation

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Use Anti-MAGOH Antibody, clone 21B12 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect MAGOH also known as mago-nashi (Drosophila) homolog proliferation-associated.
Key Applications	Western Blotting Immunoprecipitation Immunocytochemistry
Application Notes	Immunoprecipitation: Cited in Diem, MD et al. (2007) Nat. Struct.Mol. Biol. 14(12):1173-9 using a representative lot of this antibody. Immunocytochemistry: Cited in Diem, MD et al. (2007) Nat. Struct.Mol. Biol. 14(12):1173-9 using a representative lot of this antibody.

Applications

Application

Use Anti-MAGOH Antibody, clone 21B12 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect MAGOH also known as mago-nashi (Drosophila) homolog proliferation-associated.

Key Applications

Western Blotting
Immunoprecipitation
Immunocytochemistry

Application Notes

Immunoprecipitation:
Cited in Diem, MD et al. (2007) Nat. Struct.Mol. Biol. 14(12):1173-9 using a representative lot of this antibody.

Immunocytochemistry:
Cited in Diem, MD et al. (2007) Nat. Struct.Mol. Biol. 14(12):1173-9 using a representative lot of this antibody.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human MAGOH.
Epitope	Unknown
Clone	21B12
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes MAGOH.
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002361.1
Entrez Gene Summary	Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq].
Gene Symbol	MAGOH MAGOHA
Purification Method	Protein G Purified
UniProt Number	P61326
UniProt Summary	FUNCTION: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. SUBUNIT STRUCTURE: Heterodimer with RBM8A. Part of the EJC core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent exon junction complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, NCBP1, NCBP2, PNN, RBM8A, RNPS1, SRRM1, NXF1, SAP18, UPF3B, UPF2 and THOC4. Interacts with WIBG/PYM; the interaction is direct and leads to dissociate the EJC from spliced mRNAs. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note: Detected in granule-like structures in the dendroplasm by similarity. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles. TISSUE SPECIFICTY: Ubiquitous. SEQUENCE SIMILARITIES: Belongs to the mago nashi family.
Molecular Weight	~ 17 kDa

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to human MAGOH.

Epitope

Unknown

Clone

21B12

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes MAGOH.

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_002361.1

Entrez Gene Summary

Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. [provided by RefSeq].

Gene Symbol

MAGOH
MAGOHA

Purification Method

Protein G Purified

UniProt Number

P61326

UniProt Summary

FUNCTION: Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Remains associated with the mRNA after its export to the cytoplasm and require translation of the mRNA for removal. The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.

SUBUNIT STRUCTURE: Heterodimer with RBM8A. Part of the EJC core complex that contains CASC3, EIF4A3, MAGOH and RBM8A. Found in a mRNA splicing-dependent exon junction complex (EJC), at least composed of ACIN1, CASC3, EIF4A3, MAGOH, NCBP1, NCBP2, PNN, RBM8A, RNPS1, SRRM1, NXF1, SAP18, UPF3B, UPF2 and THOC4. Interacts with WIBG/PYM; the interaction is direct and leads to dissociate the EJC from spliced mRNAs. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.

SUBCELLULAR LOCATION: Nucleus. Nucleus speckle. Cytoplasm. Note: Detected in granule-like structures in the dendroplasm by similarity. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.

TISSUE SPECIFICTY: Ubiquitous.

SEQUENCE SIMILARITIES: Belongs to the mago nashi family.

Molecular Weight

~ 17 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected MAGOH on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MAGOH on 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-MAGOH, clone 21B12 (mouse monoclonal)

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