Anti-B7-H3 (CD276), clone MJ8 (rat monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, FC, IHC	R	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, FC, IHC

Purified

Monoclonal Antibody

Description
Catalogue Number	04-1501
Description	Anti-B7-H3 (CD276) Antibody, clone MJ8
Alternate Names	B7 homolog 3 CD276 antigen CD276 molecule Costimulatory molecule
Background Information	B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

Description

Catalogue Number

04-1501

Description

Anti-B7-H3 (CD276) Antibody, clone MJ8

Alternate Names

B7 homolog 3
CD276 antigen
CD276 molecule
Costimulatory molecule

Background Information

B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

Product Information
Format	Purified
Control	Human placenta tissue lysate
Presentation	Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Purified

Control

Human placenta tissue lysate

Presentation

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.
Key Applications	Western Blotting Flow Cytometry Immunohistochemistry
Application Notes	Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008). Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).

Applications

Application

Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.

Key Applications

Western Blotting
Flow Cytometry
Immunohistochemistry

Application Notes

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008).

Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).

Biological Information
Immunogen	Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.
Epitope	Extracellular Domain
Clone	MJ8
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rat
Specificity	This antibody recognizes the extracellular domain of B7-H3 (CD276).
Isotype	IgG2bκ
Species Reactivity	Human Mouse
Species Reactivity Note	Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_598744.1
Gene Symbol	CD276 4Ig-B7-H3 B7-H3 B7H3
Purification Method	Protein G Purified
UniProt Number	Q8VE98
UniProt Summary	FUNCTION: Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Play a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells. SUBUNIT STRUCTURE: Interacts with TREML2 and this interaction enhances T-cell activation By similarity. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Probable. TISSUE SPECIFICITY: Ubiquitous. DEVELOPMENTAL STAGE: Highly expressed in developing bones during embryogenesis and expression increases as osteoblast precursor cells differentiate into mature osteoblasts. INDUCTION: Up-regulated in cells mediating rejection of mouse transplant. DISRUPTION PHENOTYPE: Mice display a lower bone mineral density in cortical bones with femurs more susceptible to bone fracture, but do not exhibit any important skeletal abnormalities. Calvarial cells reveal normal number of osteoblast precursor cells with adequate proliferative capacity, but have impaired osteogenic differentiation. Furthermore, following cardiac transplantation, they display permanent survival under rapamycin regimen and cardiac transplants also show markedly decreased production of key cytokine and chemokine. The incidence of chronic transplant rejection is also inhibited. Mice also develop more severe airway inflammation and experimental autoimmune encephalomyelitis earlier than wild-type littermates as well as accumulate higher concentrations of autoantibodies to DNA. MISCELLANEOUS: Gene transfer of B7-H3 leads to complete regression of 50 per cent tumors, or significantly slows tumor growth. In primates, B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species. SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. BTN/MOG family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Molecular Weight	~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.

Biological Information

Immunogen

Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.

Epitope

Extracellular Domain

Clone

MJ8

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rat

Specificity

This antibody recognizes the extracellular domain of B7-H3 (CD276).

Isotype

IgG2bκ

Species Reactivity

Human
Mouse

Species Reactivity Note

Demonstrated to react with human.
Predicted to react with mouse based on 100% sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_598744.1

Gene Symbol

CD276
4Ig-B7-H3
B7-H3
B7H3

Purification Method

Protein G Purified

UniProt Number

Q8VE98

UniProt Summary

FUNCTION: Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Play a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.

SUBUNIT STRUCTURE: Interacts with TREML2 and this interaction enhances T-cell activation By similarity.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein Probable.

TISSUE SPECIFICITY: Ubiquitous.

DEVELOPMENTAL STAGE: Highly expressed in developing bones during embryogenesis and expression increases as osteoblast precursor cells differentiate into mature osteoblasts.

INDUCTION: Up-regulated in cells mediating rejection of mouse transplant.

DISRUPTION PHENOTYPE: Mice display a lower bone mineral density in cortical bones with femurs more susceptible to bone fracture, but do not exhibit any important skeletal abnormalities. Calvarial cells reveal normal number of osteoblast precursor cells with adequate proliferative capacity, but have impaired osteogenic differentiation. Furthermore, following cardiac transplantation, they display permanent survival under rapamycin regimen and cardiac transplants also show markedly decreased production of key cytokine and chemokine. The incidence of chronic transplant rejection is also inhibited. Mice also develop more severe airway inflammation and experimental autoimmune encephalomyelitis earlier than wild-type littermates as well as accumulate higher concentrations of autoantibodies to DNA.

MISCELLANEOUS: Gene transfer of B7-H3 leads to complete regression of 50 per cent tumors, or significantly slows tumor growth.

In primates, B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.

SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. BTN/MOG family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Molecular Weight

~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human placenta tissue lysate. Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-B7-H3 (CD276), clone MJ8 (rat monoclonal)

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