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Anti-Phosphotyrosine, recombinant clone 4G10®, biotin conjugate

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号:16-204

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
A WB M Biotin Monoclonal Antibody
Description
Catalogue Number 16-204
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
Overview Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate

Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Background Information Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
References
Product Information
Format Biotin
Control
  • A431 carcinoma cells.
    Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Presentation Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Applications
Application Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogen Phosphotyramine-KLH
Epitope N/A
Clone 4G10®
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Tyrosine-phosphorylated proteins from all species.
Isotype IgG2bκ
Species Reactivity
  • All
Antibody Type Monoclonal Antibody
Modifications
  • Phosphorylation
Purification Method Protein G Purified
Molecular Weight Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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