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Anti-phospho-Ser/Thr-Pro, MPM-2, Cy5 Conjugate

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货号：16-220

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Vrt IF, WB, ICC M Cy5 Monoclonal Antibody
Description
Catalogue Number 16-220
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate
Overview This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Background Information Progression of cells from interphase to mitosis involves alterations in cell structures and activities. The transition from G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures. Also, known as Forkhead-related protein FKHL16 or Hepatocyte nuclear factor 3 forkhead homolog 11.
References
Product Information
Format Cy5
Control
  • Colcemid-treated HeLa cell lysates
Presentation 100μg Cy5-conjugated mouse IgG1 in 200μl of PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide.
Applications
Application Anti-phospho-Ser/Thr-Pro Antibody, MPM-2, Cy5 conjugate is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in IF, WB, ICC.
Key Applications
  • Immunofluorescence
  • Western Blotting
  • Immunocytochemistry
Biological Information
Immunogen Mitotic human HeLa cell lysate
Host Mouse
Specificity Recognizes a phosphorylated epitope (phospho-[Ser/Thr]Pro) found in phosphoproteins such as MAP2, HSP70, cdc25, and DNA topoisomerase IIα, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type.
Isotype IgG1
Species Reactivity
  • Vertebrates
Antibody Type Monoclonal Antibody
Modifications
  • Phosphorylation
Purification Method Protein G Purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot on colcemid-treated HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at 4°C from date of shipment
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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