Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IP	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

WB, IP

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-843
Replaces	04-1124
Description	Anti-SIRT2 Antibody
Alternate Names	SIR2-like protein 2 silencing information regulator 2-like silent information regulator 2 sir2-related protein type 2 sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2 sirtuin 2 sirtuin type 2
Background Information	Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.

Description

Catalogue Number

09-843

Replaces

04-1124

Description

Anti-SIRT2 Antibody

Alternate Names

SIR2-like protein 2
silencing information regulator 2-like
silent information regulator 2
sir2-related protein type 2
sirtuin (silent mating type information regulation 2
homolog) 2 (S. cerevisiae)
sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2
sirtuin 2
sirtuin type 2

Background Information

Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.

Product Information
Format	Affinity Purified
Control	Human brain tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

Human brain tissue lysate

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Western Blot (SNAP ID) Analysis: 0.25 µg/mL from a representative lot detected SIRT2 on 10 µg of human brain tissue lysate. Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated SIRT2 in human brain lysate.

Applications

Application

Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Western Blot (SNAP ID) Analysis: 0.25 µg/mL from a representative lot detected SIRT2 on 10 µg of human brain tissue lysate.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated SIRT2 in human brain lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.
Epitope	Mature protein
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes the mature protein of SIRT2.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_036369
Entrez Gene Summary	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
Gene Symbol	SIR2 SIR2-like SIR2L SIR2L2
Purification Method	Affinity Purfied
UniProt Number	Q8IXJ6
UniProt Summary	FUNCTION: NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit. ENZYME REGULATION: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. SUBUNIT STRUCTURE: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules. TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. DEVELOPMENTAL STAGE: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. PTM: Phosphorylated at the G2/M transition of the cell cycle. SEQUENCE SIMILARITIES: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain. SEQUENCE CAUTION: The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.
Molecular Weight	Observed molecular weight is ~39 kDa. Multiple isoforms around 40 kDa may be observed.

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.

Epitope

Mature protein

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes the mature protein of SIRT2.

Species Reactivity

Human
Mouse

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_036369

Entrez Gene Summary

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.

Gene Symbol

SIR2
SIR2-like
SIR2L
SIR2L2

Purification Method

Affinity Purfied

UniProt Number

Q8IXJ6

UniProt Summary

FUNCTION: NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.

CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.

COFACTOR: Binds 1 zinc ion per subunit.

ENZYME REGULATION: Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide.

SUBUNIT STRUCTURE: Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton.

SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Note: Colocalizes with microtubules.

TISSUE SPECIFICITY: Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network.

DEVELOPMENTAL STAGE: Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome.

PTM: Phosphorylated at the G2/M transition of the cell cycle.

SEQUENCE SIMILARITIES: Belongs to the sirtuin family.

Contains 1 deacetylase sirtuin-type domain.

SEQUENCE CAUTION: The sequence AAF67015.1 differs from that shown. Reason: Frameshift at several positions.

Molecular Weight

Observed molecular weight is ~39 kDa. Multiple isoforms around 40 kDa may be observed.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in human brain tissue lysate. Western Blot Analysis: 0.125 µg/mL of this antibody detected SIRT2 on 10 µg of human brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.125 µg/mL of this antibody detected SIRT2 on 10 µg of human brain tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-SIRT2 (C-term), clone EP1668Y, Rabbit Monoclonal

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