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Anti-Cortactin (p80/85), clone 4F11

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号:16-229

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Av, B, H, Ht, M, R FC, IF, WB, ICC M AlexaFluor®555 Monoclonal Antibody
Description
Catalogue Number 16-229
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor® 555
References
Product Information
Format AlexaFluor®555
Applications
Application Anti-Cortactin (p80/85) Antibody, clone 4F11, Alexa Fluor 555 detects level of Cortactin (p80/85) & has been published & validated for use in FC, IF, WB & IC.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Western Blotting
  • Immunocytochemistry
Biological Information
Immunogen Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src
Clone clone 4F11
Host Mouse
Specificity Recognizes Cortactin in rodent and human cells
Isotype IgG1
Species Reactivity
  • Avian
  • Bovine
  • Human
  • Hamster
  • Mouse
  • Rat
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
Gene Symbol
  • CTTN
  • EMS1
  • Amplaxin
  • 1110020L01Rik
  • FLJ34459
Modifications
  • Phosphorylation
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
SIZE: 550 amino acids; 61636 Da
SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).
SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain.
Molecular Weight Mr 80kDa and in avian cells, Mr 80kDa and 85kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by immunoblot.
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at 4°C from date of shipment
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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