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Qiagen 凯杰 Biosharp Omega

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Anti-phospho-MKK7/SKK4 (THR275)

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号:36-013

牌:Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB Rb Purified Polyclonal Antibody
Description
Catalogue Number 36-013
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-MKK7/SKK4 (Thr275) Antibody
References
Product Information
Format Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application This Anti-phospho-MKK7/SKK4 (Thr275) Antibody is validated for use in WB for the detection of phospho-MKK7/SKK4 (Thr275).
Key Applications
  • Western Blotting
Biological Information
Immunogen peptide corresponding to amino acids surrounding phosphorylated threonine 275 of human MKK7/SKK4
Host Rabbit
Specificity MKK7/SKK4
Isotype IgG
Species Reactivity
  • Human
Species Reactivity Note Cross-reactivity with mouse expected due to sequence homology
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined.
Gene Symbol
  • MAP2K7
  • MKK7
  • PRKMK7
  • MEK7
  • MAPKK7
  • JNKK2
  • Jnkk2
Modifications
  • Phosphorylation
Purification Method Protein A Purfied
UniProt Number
UniProt Summary FUNCTION: SwissProt: O14733 # Stress activated, dual specificity kinase that activates the JUN kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3.
COFACTOR: Magnesium.
SIZE: 419 amino acids; 47485 Da
SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity).
TISSUE SPECIFICITY: Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle.
PTM: Activated by phosphorylation on Ser/Thr.
SIMILARITY: SwissProt: O14733 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight 47kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on recombinant MKK7β1, unactive (14-542) that had been phosphorylated by MEKK1 (14-196)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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