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Anti-phospho-Src (Tyr416), clone 9A6 (mouse monoclonal IgG3 )

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货号：05-677

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP M Purified Monoclonal Antibody
Description
Catalogue Number 05-677
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-phospho-Src (Tyr416) Antibody, clone 9A6
Overview Specific src isoform is unknown
Alternate Names
  • proto-oncogene tyrosine-protein kinase SRC
  • protooncogene SRC
  • Rous sarcoma, tyrosine kinase pp60c-src
  • tyrosine-protein kinase SRC-1
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Background Information Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
References
Product Information
Format Purified
Control
  • Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Presentation Purified mouse monoclonal IgG3 lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT). Lyophilized. Store at -20ºC.
Applications
Application Detect phospho-Src (Tyr416) using this Anti-phospho-Src (Tyr416) Antibody, clone 9A6 validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Immunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP.
Biological Information
Immunogen KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.
Clone clone 9A6
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.
Isotype IgG3
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • SRC
  • c-src
  • p60-Src
  • SRC1
  • c-SRC
  • ASV
  • pp60c-src
Modifications
  • Phosphorylation
Purification Method Thiophilic adsoption and size exclusion chromatography
UniProt Number
UniProt Summary SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight 60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.

Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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