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Anti-RhoE/Rnd3, clone 4 (mouse monoclonal)

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货号：05-723

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M ICC, WB M Ascites Monoclonal Antibody
Description
Catalogue Number 05-723
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-RhoE/Rnd3 Antibody, clone 4
Alternate Names
  • MemB protein
  • Rho family GTPase 3
  • ras homolog gene family, member E
  • small GTP binding protein Rho8
Background Information RhoE, also known as Rnd3, (Rho family GTPase 3) is a small G-protein that is a member of the Rnd family of Rho GTPases, which is a part of the Ras superfamily. The activity of Rnd1, Rnd2, and Rnd3/RhoE are not activated by the traditional conformational change of the GTP binding like other Rho family members. As the name dictates, Rnd proteins were associated with making a cell “round”. Like other small G-proteins, the do bind GTP, but unlike other G-proteins, they do not hydrolyze GTP to GDP. The actions of RhoE are associated with the actin cytoskeletal organization in response to external stimuli. RhoE acts antagonistically to RhoA in inducing loss of stress fiber formation, in part by binding to the RhoA downstream kinase Rho-associated kinase (ROCK1). This prevents ROCK1 activation and inhibits downstream signaling. RhoE does go through the post-translation modification of farnylation which enables it to localize and bind to various internal membranes. RhoE is also known to effect cycle progression.
References
Product Information
Format Ascites
Control
  • Included Positive Antigen Control: Catalog #12-305, 3T3/A31 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Presentation Mouse monoclonal ascites IgG in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC.
Applications
Application Anti-RhoE/Rnd3 Antibody, clone 4 is an antibody against RhoE/Rnd3 for use in IC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application Notes Immunocytochemistry:
A 1:50 dilution of this antibody has been reported by an independent laboratory to show positive immunostaining for RhoE/Rnd3 in 3T3/A31 cells fixed with paraformaldehyde and permeabilized by Triton X-100 (Riento, K., 2003).
Biological Information
Immunogen Full length fusion protein corresponding to residues 1-244 of mouse RhoE/Rnd3. Clone 4.
Clone 4
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes RhoE/Rnd3, Mr 29 kDa. Weakly cross-reacts with Rnd1, Mr 27 kDa.
Isotype IgG
Species Reactivity
  • Human
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM
Gene Symbol
  • RND3
  • RHOE
  • RhoE
  • Rho8
  • memB
  • ARHE
  • RHO8
  • Rnd3
Purification Method Unpurified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P61587 # Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
SIZE: 244 amino acids; 27368 Da
SUBUNIT: Binds ROCK1 (By similarity). Interacts with UBXD5.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: SwissProt: P61587 ## Belongs to the small GTPase superfamily. Rho family.
Molecular Weight RhoE/Rnd3 (~29 kDa); Rnd1 (~27 kDa).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.

Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected RhoE/Rnd3 in RIPA lysates from 3T3/A31 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size 200 µL
Transport Information
Supplemental Information
Specifications

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