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Anti-53BP1, clone BP13 Monoclonal Antibody

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货号：05-726

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H IP, WB, ICC M Purified Monoclonal Antibody
Description
Catalogue Number 05-726
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-53BP1 Antibody, clone BP13
References
Product Information
Format Purified
Presentation 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Applications
Application Detect 53BP1 with Anti-53BP1 Antibody, clone BP13 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB, ICC.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Application Notes Does not cross-react with mouse.
Biological Information
Immunogen Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1
Clone clone BP13
Host Mouse
Specificity 53BP1
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TP53BP1
  • 53BP1
  • p53BP1
  • p202
  • FLJ41424
  • MGC138366
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q12888 # May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Binds to sites of DNA damage. Plays a role in the response to DNA damage.
SIZE: 1972 amino acids; 213574 Da
SUBUNIT: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H3 that has been methylated on 'Lys-79'. Does not bind unmethylated histone H3.
SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks.
PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. & Phosphorylated in an ATM-dependent manner in response to DNA damage.
SIMILARITY: SwissProt: Q12888 ## Contains 2 BRCT domains.
Molecular Weight ~250kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on lysates from HeLa cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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