Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE clone 69-10C-2-10C-18

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC	Rb	Phycoerythrin	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Phycoerythrin

Monoclonal Antibody

Description
Catalogue Number	FCMAB140P
Trade Name	Milli-Mark
Description	Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18
Alternate Names	B-cell CLL/lymphoma 2 B-cell lymphoma protein 2
Background Information	The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.

Description

Catalogue Number

FCMAB140P

Trade Name

Milli-Mark

Description

Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18

Alternate Names

B-cell CLL/lymphoma 2
B-cell lymphoma protein 2

Background Information

The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.

Product Information
Format	Phycoerythrin
Control	Jurkat cells.
Presentation	Purified rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Product Information

Format

Phycoerythrin

Control

Jurkat cells.

Presentation

Purified rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Applications
Application	Detect phospho-Bcl-2 (Ser70) using this Milli-Mark Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in FC.
Key Applications	Flow Cytometry

Applications

Application

Detect phospho-Bcl-2 (Ser70) using this Milli-Mark Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in FC.

Key Applications

Flow Cytometry

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Bcl-2 phosphorylated at Ser70.
Epitope	Ser70
Clone	69-10C-2-10C-18
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes phosphorylated Bcl-2 at Ser70.
Isotype	IgG
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000624.2
Entrez Gene Summary	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
Gene Symbol	BCL2 Bcl-2
Purification Method	Protein A Purfied
UniProt Number	P10415
UniProt Summary	FUNCTION: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). SUBUNIT STRUCTURE: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity By similarity. Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. TISSUE SPECIFICTY: Expressed in a variety of tissues. DOMAIN: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1. PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) By similarity. Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. INVOLVEMENT IN DISEASE: A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. SEQUENCE SIMLARITIES: Belongs to the Bcl-2 family.
Molecular Weight	26 kDa Observed

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human Bcl-2 phosphorylated at Ser70.

Epitope

Ser70

Clone

69-10C-2-10C-18

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes phosphorylated Bcl-2 at Ser70.

Isotype

IgG

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_000624.2

Entrez Gene Summary

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]

Gene Symbol

BCL2
Bcl-2

Purification Method

Protein A Purfied

UniProt Number

P10415

UniProt Summary

FUNCTION: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

SUBUNIT STRUCTURE: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity By similarity. Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.

SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

TISSUE SPECIFICTY: Expressed in a variety of tissues.

DOMAIN: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.

PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) By similarity.

Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. INVOLVEMENT IN DISEASE: A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

SEQUENCE SIMLARITIES: Belongs to the Bcl-2 family.

Molecular Weight

26 kDa Observed

Product Usage Statements
Quality Assurance	Evaluated by flow cytometry using Jurkat cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by flow cytometry using Jurkat cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Storage and Shipping Information

Storage Conditions

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Packaging Information
Material Size	100 tests

Packaging Information

Material Size

100 tests

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE clone 69-10C-2-10C-18

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