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Anti-Neurogenin-3 (rabbit polyclonal)

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号:AB10535

牌:Millipore 密理博

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M WB, IHC Rb Serum Polyclonal Antibody
Description
Catalogue Number AB10535
Description Anti-Neurogenin-3 (rabbit) Antibody
Alternate Names
  • Neurogenin 3
  • NGN3
  • Neurogenin3
Background Information Neurogenins are a family of bHLH transcription factors involved in specifying neuronal differentiation. They are related to Drosophila atonal. The neurogenins (ngns) make up one of these atonal-related gene families. In neural crest cells, the atonal-related neurogenin family is particularly important for the sensory lineage (Neurogenins Are Essential for the Formation of dorsal root ganglia), while the achaete-scute homologue ash1 (Mash1) is important for aspects of autonomic neurogenesis (mash1 is essential for noradrenergic differentiation). Neurogenin-3 also promotes pancreatic development.
References
Product Information
Format Serum
Control
  • mNgn3 transfected and wildtype rabbit reticulocyte lysates
Presentation Unpurified rabbit polyclonal serum containing 0.05% sodium azide.
Applications
Application Detect Neurogenin-3 (rabbit) using this Anti-Neurogenin-3 (rabbit) Antibody validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Neurogenin-3 in mouse embryo E12.5+ tissue.
Biological Information
Immunogen GST-tagged recombinant protein corresponding to the N-terminus of mouse Neurogenin-3.
Epitope N-terminus
Host Rabbit
Specificity This antibody recognizes Neurogenin-3 at the N-terminus.
Isotype IgG
Species Reactivity
  • Mouse
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEUROG3
  • ATOH5
  • Atoh5
  • ngn3
  • NGN3
  • MATH4B
  • Math4B
Purification Method Unpurified
UniProt Number
UniProt Summary FUNCTION: Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.

SUBUNIT STRUCTURE: Efficient DNA binding requires dimerization with another bHLH protein.

SUBCELLULAR LOCATION: Nucleus Potential.

SEQUENCE SIMILARITIES: Contains 1 basic helix-loop-helix (bHLH) domain.
Molecular Weight 32 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in mNgn3 transfected and wildtype rabbit reticulocyte lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Neurogenin-3 on 10 µg of mNgn3 transfected rabbit reticulocyte lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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