Anti-phospho-mTOR (Ser2448) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-213
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-mTOR (Ser2448) Antibody
Alternate Names	FRAP1 FRAP FRAP2 RAFT1 RAPT1 FK506 binding protein 12-rapamycin associated protein 1
Background Information	mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

Description

Catalogue Number

09-213

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-mTOR (Ser2448) Antibody

Alternate Names

FRAP1
FRAP
FRAP2
RAFT1
RAPT1
FK506 binding protein 12-rapamycin associated protein 1

Background Information

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

Product Information
Format	Affinity Purified
Control	EGF-treated and untreated A431 cell lysate.
Presentation	Purified rabbit polyclonal IgG in storage buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

EGF-treated and untreated A431 cell lysate.

Presentation

Purified rabbit polyclonal IgG in storage buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	This Anti-mTOR Antibody is validated for use in WB for the detection of phospho-mTOR (Ser2448).
Key Applications	Western Blotting

Applications

Application

This Anti-mTOR Antibody is validated for use in WB for the detection of phospho-mTOR (Ser2448).

Key Applications

Western Blotting

Biological Information
Immunogen	Amino acids encompassing and including phosphorylated Ser2448 of human mTOR
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes mTOR when phosphorylated on Ser2448
Species Reactivity	Human
Species Reactivity Note	Tested to react with Human. Predicted to cross react with rat, mouse, sheep & Rhesus Macaque based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004949
Entrez Gene Summary	The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
Gene Symbol	FRAP1 RAPT1 mTOR FRAP MTOR FLJ44809 FRAP2 RAFT1 FRAP1 FK506 binding protein 12-rapamycin associated protein 1 mammalian target of rapamycin
Modifications	Phosphorylation
UniProt Number	P42345
UniProt Summary	FUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. SIZE: 2549 amino acids; 288892 Da SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex. TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis. SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
Molecular Weight	~289 kDa observed. An uncharacterized band may be obsereved at ~59 kDa.

Biological Information

Immunogen

Amino acids encompassing and including phosphorylated Ser2448 of human mTOR

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes mTOR when phosphorylated on Ser2448

Species Reactivity

Human

Species Reactivity Note

Tested to react with Human.
Predicted to cross react with rat, mouse, sheep & Rhesus Macaque based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_004949

Entrez Gene Summary

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.

Gene Symbol

FRAP1
RAPT1
mTOR
FRAP
MTOR
FLJ44809
FRAP2
RAFT1
FRAP1 FK506 binding protein 12-rapamycin associated protein 1
mammalian target of rapamycin

Modifications

Phosphorylation

UniProt Number

P42345

UniProt Summary

FUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
SIZE: 2549 amino acids; 288892 Da
SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.

Molecular Weight

~289 kDa observed.
An uncharacterized band may be obsereved at ~59 kDa.

Product Usage Statements
Quality Assurance	Evaluated by western blot on EGF-treated and untreated A431 cell lysate. Western Blot Analysis: This antibody detected mTOR phosphorylated on Ser2448 in EGF-treated and untreated A431 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by western blot on EGF-treated and untreated A431 cell lysate.

Western Blot Analysis:
This antibody detected mTOR phosphorylated on Ser2448 in EGF-treated and untreated A431 cell lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of shipment.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of shipment.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-mTOR (Ser2448) (rabbit polyclonal)

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