Anti-TATA-box-binding protein, clone SL30-3-563 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, ChIP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

WB, ChIP

Purified

Monoclonal Antibody

Description
Catalogue Number	05-1531
Description	Anti-TATA-box-binding protein Antibody, clone SL30-3-563
Alternate Names	TATA box binding protein Transcription initiation factor TFIID TBP subunit TATA-binding factor TATA sequence-binding protein TATA-box binding protein N-terminal domain TATA-box factor
Background Information	The TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.

Description

Catalogue Number

05-1531

Description

Anti-TATA-box-binding protein Antibody, clone SL30-3-563

Alternate Names

TATA box binding protein
Transcription initiation factor TFIID TBP subunit
TATA-binding factor
TATA sequence-binding protein
TATA-box binding protein N-terminal domain
TATA-box factor

Background Information

The TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

HeLa cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Applications
Application	Use Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initiation factor TFIID TBP subunit.
Key Applications	Western Blotting Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)

Applications

Application

Use Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initiation factor TFIID TBP subunit.

Key Applications

Western Blotting
Chromatin Immunoprecipitation (ChIP)

Application Notes

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.
Epitope	N-terminus
Clone	SL30-3-563
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes TATA-box-binding protein at the N-terminus.
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_003185
Entrez Gene Summary	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Gene Symbol	GTF2D TFIID TBP GTF2D SCA17 TF2D
Purification Method	Protein G
UniProt Number	P20226
UniProt Summary	FUNCTION: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. SUBUNIT STRUCTURE: Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1 By similarity. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Widely expressed, with levels highest in the testis and ovary. POLYMORPHISM: The poly-Gln region of TBP is highly polymorphic (25 to 42 repeats) in normal individuals and is expanded to about 47-63 repeats in spinocerebellar ataxia 17 (SCA17) patients. INVOLVEMENT IN DISEASE: Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. SEQUENCE SIMILARITIES: Belongs to the TBP family.
Molecular Weight	~38 kDa observed.

Biological Information

Immunogen

GST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.

Epitope

N-terminus

Clone

SL30-3-563

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes TATA-box-binding protein at the N-terminus.

Isotype

IgG1κ

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Demonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_003185

Entrez Gene Summary

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Gene Symbol

GTF2D
TFIID
TBP
GTF2D
SCA17
TF2D

Purification Method

Protein G

UniProt Number

P20226

UniProt Summary

FUNCTION: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

SUBUNIT STRUCTURE: Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1 By similarity. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Widely expressed, with levels highest in the testis and ovary.

POLYMORPHISM: The poly-Gln region of TBP is highly polymorphic (25 to 42 repeats) in normal individuals and is expanded to about 47-63 repeats in spinocerebellar ataxia 17 (SCA17) patients.

INVOLVEMENT IN DISEASE: Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.

SEQUENCE SIMILARITIES: Belongs to the TBP family.

Molecular Weight

~38 kDa observed.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-TATA-box-binding protein, clone SL30-3-563 (mouse monoclonal)

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