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Anti-Ninein, clone 79-160 (mouse monoclonal)

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货号：MABT29

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP M Purified Monoclonal Antibody
Description
Catalogue Number MABT29
Description Anti-Ninein Antibody, clone 79-160-7
Alternate Names
  • ninein (GSK3B interacting protein)
  • Glycogen synthase kinase 3 beta-interacting protein
  • ninein centrosomal protein
  • GSK3B-interacting protein
Background Information Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.
References
Product Information
Format Purified
Control
  • Hek293 cell lysate
Presentation Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.

Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).
Biological Information
Immunogen Recombinant protein corresponding to human Ninein.
Clone 79-160-7
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Isotype IgG2aκ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq].
Gene Symbol
  • ninein
  • hNinein
  • NIN
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules.

SUBUNIT STRUCTURE: Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3-beta.

SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton › centrosome. Note: Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization.

TISSUE SPECIFICITY: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5.

DEVELOPMENTAL STAGE: In interphase cells, it is localized in the centrosome. Decreases in metaphase and anaphase and reappears in telophase.

DOMAIN: The coiled coil region from Asn-1611 to Pro-1693 is necessary and sufficient for the targeting to centrosome.

PTM: Phosphorylated by Aurora A (AIK) and PKA kinases but not CK2 or Aurora B (AIM). Phosphorylated upon DNA damage, probably by ATM or ATR.

MISCELLANEOUS: Antibodies against NIN are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins.

SEQUENCE SIMILARITIES: Contains 5 EF-hand domains.

SEQUENCE CAUTION: The sequence AAH65521.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

The sequence BAB13391.2 differs from that shown. Reason: Erroneous initiation.

The sequence BAB15640.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight ~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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