[关闭]
您好！欢迎来到炼石商城   请登录  注册    兼职小伙伴  
我的购物车
0
Qiagen 凯杰 Biosharp Omega

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准
收藏

Anti-RhoGAP p190, clone D2D6

市场价：
0.00
促销价：
¥0.00

货号：05-378

品牌：Millipore 密理博

配送至：

支付： 账期 货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次
RNeasy Mini Kit (50) 50次
3720.00
Plasmid Mini Kit I(200) 200次
Plasmid Mini Kit I(200) 200...
576.00
10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱
10ul吸头，透明，袋装，可灭菌...
85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10
QIAfilter Plasmid Maxi Kit (...
3000.00
QIAamp DNA Blood Midi Kit (20) 20次
QIAamp DNA Blood Midi Kit (2...
2680.00
QIAamp UCP Pathogen Mini Kit (50)
QIAamp UCP Pathogen Mini Kit...
4112.00
Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Mk IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number 05-378
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-RhoGAP p190 Antibody, clone D2D6
Alternate Names
  • CDC42 GTPase-activating protein
  • GTPase-activating protein rhoOGAP
  • Rho GTPase activating protein 1
  • Rho-related small GTPase protein activator
  • Rho-type GTPase-activating protein 1
Background Information RhoGAP p190 (Rho GTPase Activating Protein 5, ARHGAP5), like other RhoGAPs, stimulates the intrinsic GTPases activity of Rho, Rac, and Cdc42, but shows definite preference for Rho. RhoGAP p190 contains an N-terminal GTPase domain and a C-terminal RhoGAP domain. The activity of RhoGAP p190 is known to be regulated by phosphorylation, by kinases such as Src that is stimulated by growth factor signaling or cell attachment and phosphorylate RhoGAP p190 in its central region. This ultimately leads to reduced RHO-GTP and decreased stress fibers.
References
Product Information
Format Purified
Control
  • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels.
Presentation Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
Applications
Application Detect RhoGAP p190 using this Anti-RhoGAP p190 Antibody, clone D2D6 validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application Notes Immunoprecipitation:
4 μg of a previous lot immunoprecipitated RhoGAP from 500 μg of human A431 carcinoma cell RIPA lysate.
Biological Information
Immunogen GST-fusion protein to amino acids 180-610 of rat p190 RhoGAP.
Clone D2D6
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity Recognizes RhoGAP, MW ~190kDa.
Isotype IgG
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Monkey
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARHGAP1
  • RHOGAP
  • p50-RhoGAP
  • CDC42GA1
  • CDC42GAP
  • Cdc42GAP
  • p50rhoGAP
  • RHOGAP1
  • RhoGAP
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q07960 # GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.
SIZE: 439 amino acids; 50436 Da
SUBUNIT: Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with BNIPL.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: SwissProt: Q07960 ## Contains 1 CRAL-TRIO domain. & Contains 1 Rho-GAP domain.
Molecular Weight ~190 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated in immunoblot on RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts or rabbit brain membrane extract.

Western Blot Analysis:
0.5-2 μg/mL of this antibody detected RhoGAP in RIPA lysates from human A431 carcinoma cells. Previous lots of the antibody detected RhoGAP in mouse 3T3 fibroblasts and a rabbit brain membrane extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at -20ºC from date of receipt.
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！

我要咨询

咨询内容：
购物指南 免费注册 批量采购 购物流程 购物保障
配送方式 上门自提 第三方配送 炼石配送
支付方式 货到付款 账期付款
售后服务 售后政策 退款说明 售后流程 免责条款

服务热线

0771-3293894

关于我们|帮助中心|诚聘英才|炼石云实验室|联系我们|友情链接

Copyright 2014-2025 炼石云商网络科技（海南）有限公司版权所有 琼ICP备2023006525号-3