Anti-PRKDC/DNA-PK, clone Y393, Rabbit Monoclonal - 炼石商城

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Anti-PRKDC/DNA-PK, clone Y393, Rabbit Monoclonal

市场价：: 0.00

促销价：: ¥0.00

货号：04-1024

品牌：Millipore 密理博

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北京朝阳

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IHC, ICC, ELISA	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MABE629
Replaces	04-1024
Description	Anti-PRKDC, clone 3H6 Antibody
Alternate Names	DNA-dependent protein kinase catalytic subunit DNA-PK catalytic subunit DNA-PKcs PRKDC
Background Information	DNA-dependent protein kinase catalytic subunit (DNA-PK catalytic subunit or DNA-PKcs), also known as DNPK1 or p460, and encoded by the gene name PRKDC or HYRC, is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required to protect and align broken ends of DNA and V(D)J recombination. DNA-PK/PRKDC must be bound to DNA to express its catalytic properties. Autophosphorylation of DNA-PK/PRKDC correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK/PRKDC phosphorylates Hsp90, p53 and c-Jun.

References

Product Information
Format	Ascites
Presentation	Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.

Applications
Application	Anti-PRKDC, clone 3H6 Antibody is an antibody against PRKDC for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA.
Key Applications	Western Blotting Immunohistochemistry Immunocytochemistry ELISA
Application Notes	Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKDC in breast cancer, colon cancer, lung cancer, and brain tissues. Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKDC in HeLa cells. ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Biological Information
Immunogen	Purified recombinant fragment of human PRKDC expressed in E. Coli.
Clone	3H6
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG1
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Gene Symbol	PRKDC HYRC HYRC1
Purification Method	Unpurified
UniProt Number	P78527
Molecular Weight	~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HEK293 and PRKDC -- hIgGFc transfected HEK293 cell lysates. Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKDC in HEK293 and hIgGFc transfected HEK293 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

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