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Anti-Vinculin, clone V284 (mouse monoclonal IgG1 )

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货号：05-386

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
Ch, H, M, R, Rb IP, WB, IHC M Purified Monoclonal Antibody
Description
Catalogue Number 05-386
Brand Family Upstate
Trade Name
  • Upstate
Description Anti-Vinculin Antibody, clone V284
References
Product Information
Format Purified
Control
  • includes non-stimulated A431 cell lysate as a positive control, cat. no. 12-301
Presentation PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
Applications
Application Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
Biological Information
Immunogen Human Vinculin
Clone clone V284
Host Mouse
Specificity Vinculin
Isotype IgG1
Species Reactivity
  • Chicken
  • Human
  • Mouse
  • Rat
  • Rabbit
Antibody Type Monoclonal Antibody
Entrez Gene Number
Entrez Gene Summary Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater than 95% sequence identity to the chicken vinculin protein.
Gene Symbol
  • VCL
  • Metavinculin
  • OTTHUMP00000043885
  • vinculin
  • MVCL
  • Vinculin
Purification Method Protein A chromatography
UniProt Number
UniProt Summary FUNCTION: SwissProt: P18206 # Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.
SIZE: 1134 amino acids; 123799 Da
SUBUNIT: Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Note=Cytoplasmic face of adhesion plaques.
TISSUE SPECIFICITY: Metavinculin is muscle-specific.
PTM: Phosphorylated on serines, threonines and tyrosines and acylated by myristic acid and/or palmitic acid.
DISEASE:SwissProt: P18206 # Defects in VCL are a cause of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM).
SIMILARITY: SwissProt: P18206 ## Belongs to the vinculin/alpha-catenin family.
Molecular Weight 117kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 2 years at -20°C
Packaging Information
Material Size 200 µg
Transport Information
Supplemental Information
Specifications

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