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Milli-Mark™ Anti-RPA1 p70 -FITC clone RPA9, 1-30

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货号：FCMAB144F

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H FC M FITC Monoclonal Antibody
Description
Catalogue Number FCMAB144F
Trade Name
  • Milli-Mark
Description Milli-Mark Anti-RPA1 p70 -FITC Antibody, clone RPA9, 1-30
Alternate Names
  • RF-A protein 1
    RP-A p70
    Replication factor A protein 1
    Single-stranded DNA-binding protein
    replication protein A 70 kDa DNA-binding subunit
    replication protein A1 (70kD)
    replication protein A1, 70kDa
    replication protein A 70 kDa DNA-binding subunit
    REPA1
    RPA1
    HSSB.
Background Information Replication protein A (RPA) is a multisubunit complex involved in DNA mismatch repair (MMR) in association with MSH2, MLH1 , PCNA, and DNA polymerase-delta. RPA is a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA1 p70 kDa is an ssDNA binding protein shown to be phosphorylated by ATM and ATR in response to DNA damage.RPA also functions in the initiation of DNA replication and DNA unwinding at origins of replication.
References
Product Information
Format FITC
Control
  • HeLa Cells
Presentation Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Applications
Application Milli-Mark Anti-RPA1 p70 -FITC Antibody, clone RPA9, 1-30 is an antibody against RPA1 p70 for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
Immunogen His-tagged recombinant protein corresponding to human RPA1.
Clone RPA9, 1-30
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes RPA1
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RPA1
  • REPA1
  • RF-A
  • RP-A
  • RPA70
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.


SUBUNIT STRUCTURE: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.


SUBCELLULAR LOCATION: Nucleus.


PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.


SEQUENCE SIMILARITIES: Belongs to the replication factor A protein 1 family.
Molecular Weight ~68kDa Observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Tested by flow cytometry using HeLa Cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size 100 tests
Transport Information
Supplemental Information
Specifications

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