Anti-GSK3, clone 4G-1E

Species Reactivity	Key Applications	Host	Format	Antibody Type
Vrt, M, R, H, Di, Yeast (S. pombe)	ICC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

Vrt, M, R, H, Di, Yeast (S. pombe)

ICC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-412
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-GSK3 Antibody, clone 4G-1E
Alternate Names	GSK-3 alpha glycogen synthase kinase 3 alpha
Background Information	Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with ‘unspliced’ GSK-3b. GSK-3b2 is localized primarily to neuronal cell bodies, unlike unspliced GSK-3b that is also found in neuronal processes. GSK-3b plays a key inhibitory role in the Wnt signaling pathway. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in development and in maintenance of adult tissues. Abnormalities in Wnt signaling are reported to promote both human dgenerative diseases and cancer.

Description

Catalogue Number

05-412

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-GSK3 Antibody, clone 4G-1E

Alternate Names

GSK-3 alpha
glycogen synthase kinase 3 alpha

Background Information

Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with ‘unspliced’ GSK-3b. GSK-3b2 is localized primarily to neuronal cell bodies, unlike unspliced GSK-3b that is also found in neuronal processes. GSK-3b plays a key inhibitory role in the Wnt signaling pathway. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in development and in maintenance of adult tissues. Abnormalities in Wnt signaling are reported to promote both human dgenerative diseases and cancer.

Product Information
Format	Purified
Control	Jurkate cell lysate, 293T cell extract, rabbit spleen fibroblasts Included Positive Antigen Control: Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.
Presentation	Protein G purified mouse IgG1 in storage buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Product Information

Format

Purified

Control

Jurkate cell lysate, 293T cell extract, rabbit spleen fibroblasts

Included Positive Antigen Control:
Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels.

Presentation

Protein G purified mouse IgG1 in storage buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Applications
Application	Anti-GSK3 Antibody, clone 4G-1E detects level of GSK3 & has been published & validated for use in IC & WB.
Key Applications	Immunocytochemistry Western Blotting
Applications Not Recommended	Immunoprecipitation
Application Notes	Immunoprecipitation: Not recommended. Immunocytochemistry: 10 µg/mL of a previous lot showed positive immunostaining for GSK3 in L6 cells fixed with acetone.

Applications

Application

Anti-GSK3 Antibody, clone 4G-1E detects level of GSK3 & has been published & validated for use in IC & WB.

Key Applications

Immunocytochemistry
Western Blotting

Applications Not Recommended

Immunoprecipitation

Application Notes

Immunoprecipitation:
Not recommended.

Immunocytochemistry:
10 µg/mL of a previous lot showed positive immunostaining for GSK3 in L6 cells fixed with acetone.

Biological Information
Immunogen	18 residue synthetic peptide (C-KQLLHGEP NVSYICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme, coupled to ovalbumin. Clone 4G-1E.
Clone	4G-1E
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	GSK3, α and β isoforms
Isotype	IgG1
Species Reactivity	Vertebrates Mouse Rat Human Dictyostelium Yeast (S. pombe)
Species Reactivity Note	Mouse, rat, S. pombe, Dictyostelium and human. Because of the extremely high conservation of this immunogen among the known enzymes of many different species, this antibody is predicted to cross-react with nearly all species homologues.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_057366
Entrez Gene Summary	Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM]
Gene Symbol	GSK3A DKFZp686D0638
Purification Method	Protein G Purified
UniProt Number	P49840
UniProt Summary	FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity). SIZE: 483 amino acids; 50981 Da SUBUNIT: Monomer (By similarity). SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein
Molecular Weight	51 kDa (α) or 46 kDa (β)

Biological Information

Immunogen

18 residue synthetic peptide (C-KQLLHGEP NVSYICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme, coupled to ovalbumin. Clone 4G-1E.

Clone

4G-1E

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

GSK3, α and β isoforms

Isotype

IgG1

Species Reactivity

Vertebrates
Mouse
Rat
Human
Dictyostelium
Yeast (S. pombe)

Species Reactivity Note

Mouse, rat, S. pombe, Dictyostelium and human. Because of the extremely high conservation of this immunogen among the known enzymes of many different species, this antibody is predicted to cross-react with nearly all species homologues.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_057366

Entrez Gene Summary

Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM]

Gene Symbol

GSK3A
DKFZp686D0638

Purification Method

Protein G Purified

UniProt Number

P49840

UniProt Summary

FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity).
SIZE: 483 amino acids; 50981 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein

Molecular Weight

51 kDa (α) or 46 kDa (β)

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot on human Jurkat cells, human A431, rat L6, rat PC12 or mouse 3T3 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected GSK3 in RIPA lysates from human Jurkat cells; previously GSK3 was detected in RIPA lysates from human A431, rat L6 and PC12, and mouse 3T3 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by immunoblot on human Jurkat cells, human A431, rat L6, rat PC12 or mouse 3T3 cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected GSK3 in RIPA lysates from human Jurkat cells; previously GSK3 was detected in RIPA lysates from human A431, rat L6 and PC12, and mouse 3T3 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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