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Qiagen 凯杰 Biosharp Omega

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Anti-Calponin-1 (C-Term), Rabbit Monoclonal, clone EP798Y

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号:04-589

牌:Millipore 密理博

账期 货到付款

EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IH(P), IF Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number ABT129
Replaces 04-589
Description Anti-Calponin-1 Antibody
Alternate Names
  • Calponin-1
  • Basic calponin
  • Calponin H1, smooth muscle
Background Information Calponin 1 is a 34 kDa protein that is predominantly found in smooth muscle tissue. It is a high-affinity actin-binding protein, but also interacts with tropomyosin, and calmodulin to modulate muscle contraction. When bound to F-actin, it inhibits the activity of the actin-activated myosin ATPase. Calponin 1 is regulated by reversible phosphorylation involving PKC and CAMKII kinases, and type 2A phosphatases. In non-muscle cells, calponin 1 may play a role in cell migration, motility, and proliferation.
References
Product Information
Format Affinity Purified
Control
  • Human aortic smooth muscle tissue
Presentation Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-Calponin-1 Antibody is validated for use in Western Blotting, IHC(P), Immunofluorescence for the detection of Calponin-1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application Notes Immunohistochemistry Analysis: A 1:1,000 dilution from a reprsesentative lot detected Calponin-1 in human seminal vesicle tissue.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Calponin-1 in smooth muscle cells from human seminal vesicle tissue.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to the C-terminus of human Calponin-1.
Epitope C-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity This antibody recognizes the C-terminus of Calponin-1.
Species Reactivity
  • Human
Species Reactivity Note Demonstrated to react with Human. Predicted to react with Bovine, Chimpanzee, and Sheep based on 100% sequence homology. Other homologies: Rat (92% sequence homology) and Mouse (83% sequence homology).
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CNN1
Purification Method Affinity Purfied
UniProt Number
Molecular Weight ~33 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in human aortic smooth muscle tissue.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Calponin-1 in human aortic smooth muscle tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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