Anti-CTLA4 (CD152), clone 9H10 (hamster monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB, FC, IP	Ht	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, FC, IP

Purified

Monoclonal Antibody

Description
Catalogue Number	04-963
Description	Anti-CTLA4 (CD152) Antibody, clone 9H10
Alternate Names	CD152 antigen Cytotoxic T-lymphocyte-associated antigen 4 celiac disease 3 cytotoxic T-lymphocyte-associated protein 4 cytotoxic T-lymphocyte-associated serine esterase-4 ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Background Information	CTLA4, Cytotoxic T lymphocyte antigen 4, also known as CD152, belongs to the family of immunoglobins. It is widely expressed on activated T lymphocytes and is involved in the maintenance of immune tolerance. CTLA4 is a homolog for CD28. They are able to bind to B7-1 and B7-2; however, CTLA4 is able to bind to both with a higher affinity. Defects in CTLA4 have been linked to Rheumatoid arthritis, Addisons disease, Graves disease and many other autoimmune diseases.

Description

Catalogue Number

04-963

Description

Anti-CTLA4 (CD152) Antibody, clone 9H10

Alternate Names

CD152 antigen
Cytotoxic T-lymphocyte-associated antigen 4
celiac disease 3
cytotoxic T-lymphocyte-associated protein 4
cytotoxic T-lymphocyte-associated serine esterase-4
ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background Information

CTLA4, Cytotoxic T lymphocyte antigen 4, also known as CD152, belongs to the family of immunoglobins. It is widely expressed on activated T lymphocytes and is involved in the maintenance of immune tolerance. CTLA4 is a homolog for CD28. They are able to bind to B7-1 and B7-2; however, CTLA4 is able to bind to both with a higher affinity. Defects in CTLA4 have been linked to Rheumatoid arthritis, Addisons disease, Graves disease and many other autoimmune diseases.

Product Information
Format	Purified
Control	Mouse spleen cell lysate
Presentation	Purified hamster monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) with 150 mM NaCl and 0.05% sodium azide.

Product Information

Format

Purified

Control

Mouse spleen cell lysate

Presentation

Purified hamster monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) with 150 mM NaCl and 0.05% sodium azide.

Applications
Application	Anti-CTLA4 (CD152) Antibody, clone 9H10 is an antibody against CTLA4 (CD152) for use in WB, FC & IP.
Key Applications	Western Blotting Flow Cytometry Immunoprecipitation
Application Notes	Flow Cytometry Analysis: Representative images from a previous lot of primary antibody that was biotinylated. Results with 04-963 are expected to be similar if used directly with an anti-Syrian hamster conjugated antibody, however because of the low expression of CTLA4 Fab fragmented goat anti-Syrian hamster biotin followed SA-fluorophore is recommended for best results. FACS analysis of ConA (24 hours) stimulated BALB/C splenocytes for surface expression of CTLA-4 (CD152). Cells were stained with 10 ug of biotin-labeled hamster anti-CTLA-4 ,clone 9H10 (open histogram) or with PE-Sav secondary antibody alone (histogram, Figure B). Note the distinct signal difference between the control (dark control histogram) and test (clear histogram). Immunoprecipitation: Clone 9H10 is widely used for immunoprecipitaiton of CTLA-4; recommended lysis buffers include 1% NP40; 0.5% triton X-100 with proteinase inhibitors; 1-5 µg of 9H10 is added to 300-500 µL of whole cell lysate or cell membrane protein preparations (250-500 mg/mL totall protein) and incubated 3-6 hours at 4°C, followed by capture with protein G beads following standard immunoprecipitation methods (Zhang, Y., et al., 1997).

Applications

Application

Anti-CTLA4 (CD152) Antibody, clone 9H10 is an antibody against CTLA4 (CD152) for use in WB, FC & IP.

Key Applications

Western Blotting
Flow Cytometry
Immunoprecipitation

Application Notes

Flow Cytometry Analysis: Representative images from a previous lot of primary antibody that was biotinylated. Results with 04-963 are expected to be similar if used directly with an anti-Syrian hamster conjugated antibody, however because of the low expression of CTLA4 Fab fragmented goat anti-Syrian hamster biotin followed SA-fluorophore is recommended for best results.
FACS analysis of ConA (24 hours) stimulated BALB/C splenocytes for surface expression of CTLA-4 (CD152). Cells were stained with 10 ug of biotin-labeled hamster anti-CTLA-4 ,clone 9H10 (open histogram) or with PE-Sav secondary antibody alone (histogram, Figure B). Note the distinct signal difference between the control (dark control histogram) and test (clear histogram).
Immunoprecipitation: Clone 9H10 is widely used for immunoprecipitaiton of CTLA-4; recommended lysis buffers include 1% NP40; 0.5% triton X-100 with proteinase inhibitors; 1-5 µg of 9H10 is added to 300-500 µL of whole cell lysate or cell membrane protein preparations (250-500 mg/mL totall protein) and incubated 3-6 hours at 4°C, followed by capture with protein G beads following standard immunoprecipitation methods (Zhang, Y., et al., 1997).

Biological Information
Immunogen	Mouse CTLA-4/human IgG1 fusion protein corresponding to residues surrounding CTLA4.
Clone	9H10
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Hamster
Specificity	This antibody recognizes CTLA4. Antibody 9H10 is specific for CTLA-4 by four criteria: (a) Reactivity against CTLA-4 Ig but not CD4 lg; (b) the ability to block CTLA-4 Ig binding to B7 transfectants; (c) the ability to stain activated T cells but not freshly isolated T cells; and (d) the ability to stain a CTLA-4 transfectant but not control transfectants (Krummel, M.F., et al., 1995).
Isotype	IgG
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_033973.2
Gene Symbol	CD CD152 CD28 CELIAC3 CTLA-4 GSE ICOS IDDM12 OTTHUMP00000163781
Purification Method	Protein G Purified
UniProt Number	P16410
UniProt Summary	FUNCTION: Possibly involved in T-cell activation. Binds to B7-1 (CD80) and B7-2 (CD86). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Widely expressed with highest levels in lymphoid tissues. INVOLVEMENT IN DISEASE: Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variation in CTLA4 may be a cause of susceptibility to Graves disease (GRD) [MIM:275000]. GRD is an autoimmune disorder causing overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to insulin-dependent diabetes mellitus type 12 (IDDM12) [MIM:601388]. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. Celiac disease [MIM:212750] is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. Genetic variations in CTLA4 are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SEQUENCE SIMILARITIES: Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Molecular Weight	~30 kDa

Biological Information

Immunogen

Mouse CTLA-4/human IgG1 fusion protein corresponding to residues surrounding CTLA4.

Clone

9H10

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Hamster

Specificity

This antibody recognizes CTLA4. Antibody 9H10 is specific for CTLA-4 by four criteria: (a) Reactivity against CTLA-4 Ig but not CD4 lg; (b) the ability to block CTLA-4 Ig binding to B7 transfectants; (c) the ability to stain activated T cells but not freshly isolated T cells; and (d) the ability to stain a CTLA-4 transfectant but not control transfectants (Krummel, M.F., et al., 1995).

Isotype

IgG

Species Reactivity

Mouse

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_033973.2

Gene Symbol

CD
CD152
CD28
CELIAC3
CTLA-4
GSE
ICOS
IDDM12
OTTHUMP00000163781

Purification Method

Protein G Purified

UniProt Number

P16410

UniProt Summary

FUNCTION: Possibly involved in T-cell activation. Binds to B7-1 (CD80) and B7-2 (CD86).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Widely expressed with highest levels in lymphoid tissues.
INVOLVEMENT IN DISEASE: Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variation in CTLA4 may be a cause of susceptibility to Graves disease (GRD) [MIM:275000]. GRD is an autoimmune disorder causing overactivity of the thyroid gland and hyperthyroidism.
Genetic variation in CTLA4 is the cause of susceptibility to insulin-dependent diabetes mellitus type 12 (IDDM12) [MIM:601388].
Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. Celiac disease [MIM:212750] is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
Genetic variations in CTLA4 are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
SEQUENCE SIMILARITIES: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Molecular Weight

~30 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in mouse spleen lysate. Western Blot Analysis: 1 µg/mL of a biotinylated version of this antibody detected CTLA4 in 10 µg of mouse spleen lysate. THIS ANTIBODY IS NO BIOTINYLATED.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in mouse spleen lysate.
Western Blot Analysis: 1 µg/mL of a biotinylated version of this antibody detected CTLA4 in 10 µg of mouse spleen lysate.
THIS ANTIBODY IS NO BIOTINYLATED.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C for 1 year from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable at 2-8°C for 1 year from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CTLA4 (CD152), clone 9H10 (hamster monoclonal)

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