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Anti-PICH, clone 142-26-3 (mouse monoclonal)

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货号：04-1540

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, IP, ICC M Purified Monoclonal Antibody
Description
Catalogue Number 04-1540
Description Anti-PICH Antibody, clone 142-26-3
Alternate Names
  • excision repair cross-complementing rodent repair deficiency, complementation group 6-like
  • excision repair cross-complementing rodent repair deficiency complementation group 6 - like
  • Tumor antigen BJ-HCC-15
  • PLK1-interacting checkpoint helicase
  • ATP-dependent helicase ERCC6-like
  • excision repair protein ERCC6-like
  • SNF2/RAD54 family protein
  • DNA excision repair protein ERCC-6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15
Background Information Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.
References
Product Information
Format Purified
Control
  • HeLa-S3 cell lysate
Presentation Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Applications
Application Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application Notes Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
Biological Information
Immunogen Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.
Epitope Unknown
Clone 142-26-3
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Mouse
Specificity This antibody recognizes Plk1-interacting checkpoint helicase.
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PICH
  • ERCC6L
  • FLJ20105
  • MGC131695
Purification Method Protein G Purified
UniProt Number
UniProt Summary FUNCTION: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.

SUBUNIT STRUCTURE; Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization.

SUBCELLULAR LOCATION: Centromere. Kinetochore. Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset.

PTM: Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.

SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 TPR repeats.

SEQUENCE CAUTION: The sequence AAM82750.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.

The sequence BAA90952.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.
Molecular Weight ~160 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in HeLa-S3 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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