Anti-Rac1, clone 23A8, agarose conjugate

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IP	M	Gel Immobilized	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

Gel Immobilized

Monoclonal Antibody

Description
Catalogue Number	16-637
Description	Anti-Rac1 Antibody, clone 23A8, Agarose conjugate
Alternate Names	Cell migration-inducing gene 5 protein Ras-like protein TC25 migration-inducing gene 5 migration-inducing protein 5 ras-related C3 botulinum toxin substrate 1 ras-related C3 botulinum toxin substrate 1 (rho family,small GTP binding protein Rac1) ras-related C3 botulinum toxin substrate 1 isoform Rac1 rho family, small GTP binding protein Rac1
Background Information	Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.

Description

Catalogue Number

16-637

Description

Anti-Rac1 Antibody, clone 23A8, Agarose conjugate

Alternate Names

Cell migration-inducing gene 5 protein
Ras-like protein TC25
migration-inducing gene 5
migration-inducing protein 5
ras-related C3 botulinum toxin substrate 1
ras-related C3 botulinum toxin substrate 1 (rho family,small GTP binding protein Rac1)
ras-related C3 botulinum toxin substrate 1 isoform Rac1
rho family, small GTP binding protein Rac1

Background Information

Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.

Product Information
Format	Gel Immobilized
Presentation	Anti-Rac1 mouse monoclonal antibody covalently coupled to 100 L of protein G agarose beads and proved as a 50% slurry suspended in PBS, containing 0.05% sodium azide as a preservative, for a total volume of 200 L. Liquid suspension.

Product Information

Format

Gel Immobilized

Presentation

Anti-Rac1 mouse monoclonal antibody covalently coupled to 100 L of protein G agarose beads and proved as a 50% slurry suspended in PBS, containing 0.05% sodium azide as a preservative, for a total volume of 200 L. Liquid suspension.

Applications
Application	Detect Rac1 using this Anti-Rac1 Antibody, clone 23A8, Agarose conjugate validated for use in IP.
Key Applications	Immunoprecipitation
Application Notes	Immunoprecipitation Analysis: This product is useful for immunoprecipitation and immunoaffinity chromatography methods. This antibody conjugate was used to immunoprecipitate (IP) Rac1 from rat brain microsomal preparation using 20L of the 50% slurry to IP Rac1 from 250 g of rat brain microsomal preparation (Cat. No. 12-144). IP sample was resolved by SDS-PAGE and detect using anti-Rac, clone 23A8 (Cat. No. 05-389).

Applications

Application

Detect Rac1 using this Anti-Rac1 Antibody, clone 23A8, Agarose conjugate validated for use in IP.

Key Applications

Immunoprecipitation

Application Notes

Immunoprecipitation Analysis:
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.

This antibody conjugate was used to immunoprecipitate (IP) Rac1 from rat brain microsomal preparation using 20L of the 50% slurry to IP Rac1 from 250 g of rat brain microsomal preparation (Cat. No. 12-144). IP sample was resolved by SDS-PAGE and detect using anti-Rac, clone 23A8 (Cat. No. 05-389).

Biological Information
Immunogen	Recombinant protein containing the full length human Rac1.
Clone	clone 23A8
Host	Mouse
Specificity	Recognizes Rac1 with a strong preference. There is a slight Rac2 cross-reactivity.
Isotype	IgG2b
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Human, Mouse, Rat. Other species have not been tested.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_008839
Entrez Gene Summary	The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Gene Symbol	MIG5 PAK1 TC-25 p21-Rac1
UniProt Number	P63000
UniProt Summary	Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Subunit structure: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH By similarity. Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sideBy similarity. Melanosome. Note= Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine By similarity. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. Domain: The effector region mediates interaction with DEF6. Sequence similarities: Belongs to the small GTPase superfamily. Rho family.
Molecular Weight	21 kDa

Biological Information

Immunogen

Recombinant protein containing the full length human Rac1.

Clone

clone 23A8

Host

Mouse

Specificity

Recognizes Rac1 with a strong preference. There is a slight Rac2 cross-reactivity.

Isotype

IgG2b

Species Reactivity

Human
Mouse
Rat

Species Reactivity Note

Human, Mouse, Rat. Other species have not been tested.

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_008839

Entrez Gene Summary

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Gene Symbol

MIG5
PAK1
TC-25
p21-Rac1

UniProt Number

P63000

UniProt Summary

Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.
Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
Subunit structure: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, BAIAP2L1, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2. Interacts with NOXA1. Interacts with ARHGEF2. Interacts with NISCH By similarity.
Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sideBy similarity. Melanosome. Note= Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine By similarity. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Domain: The effector region mediates interaction with DEF6.
Sequence similarities: Belongs to the small GTPase superfamily. Rho family.

Molecular Weight

21 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 4 months at 2-8°C from date of receipt. DO NOT FREEZE! Wash the agarose beads with an appropriate buffer prior to use to remove sodium azide.

Storage and Shipping Information

Storage Conditions

Stable for 4 months at 2-8°C from date of receipt. DO NOT FREEZE! Wash the agarose beads with an appropriate buffer prior to use to remove sodium azide.

Packaging Information
Material Size	200 µL

Packaging Information

Material Size

200 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-Rac1, clone 23A8, agarose conjugate

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