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Ral Assay Reagent (Ral BP1, agarose)

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货号：14-415

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number 14-415
Brand Family Upstate
Trade Name
  • Upstate
Description Ral Assay Reagent (Ral BP1, agarose)
Overview GST fusion-protein, corresponding to residues 397-519 of rat Ral binding protein (RalBP1) bound to agarose. Also referred to as RLIP and RLP; specifically binds to and precipitates RalA-GTP and RalB-GTP from cell lysates.
References
Product Information
Presentation 300 μg of protein in 600 μL of a 50% agarose slurry in 50 mM Tris, pH 7.5, 200 mM NaCl, 1% Triton X-100, 20 μM PMSF, 1 mM aprotinin prior to the addition of glycerol to 50%. Liquid suspension.
Applications
Key Applications
  • Affinity Binding Assay
Biological Information
Source Expressed in E. coli.
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • RALBP1
  • RLIP1
  • RIP1
  • RalBP1
  • RIP
  • RLIP76
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q15311 # Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
SIZE: 655 amino acids; 76063 Da
SUBUNIT: Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDC2 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
SIMILARITY: SwissProt: Q15311 ## Contains 1 Rho-GAP domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by Affinity Precipitation/Immunoblot Analysis
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -20°C
Packaging Information
Material Size 300 µg
Transport Information
Supplemental Information
Specifications

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