Anti-CTCF, clone 1.2.1.5.4 (mouse monoclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IP, IHC, ChIP	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, IP, IHC, ChIP

Purified

Monoclonal Antibody

Description
Catalogue Number	04-1506
Replaces	MABE547
Description	Anti-CTCF Antibody, clone 1.2.1.5.4
Alternate Names	11 zinc finger transcriptional repressor 11-zinc finger protein CCCTC-binding factor CCCTC-binding factor (zinc finger protein) CTCFL paralog transcriptional repressor CTCF
Background Information	CTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor.

Description

Catalogue Number

04-1506

Replaces

MABE547

Description

Anti-CTCF Antibody, clone 1.2.1.5.4

Alternate Names

11 zinc finger transcriptional repressor
11-zinc finger protein
CCCTC-binding factor
CCCTC-binding factor (zinc finger protein)
CTCFL paralog
transcriptional repressor CTCF

Background Information

CTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor.

Product Information
Format	Purified
Control	K562 cell lysate
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Product Information

Format

Purified

Control

K562 cell lysate

Presentation

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Applications
Application	Anti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC.
Key Applications	Western Blotting Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation (ChIP)
Application Notes	Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et al. (2005). Human Molecular Genetics. 14(7):953–965.)

Applications

Application

Anti-CTCF Antibody, clone 1.2.1.5.4 is a Mouse Monoclonal Antibody for detection of CTCF also known as 11-zinc finger protein, CCCTC-binding factor & has been validated in WB, IP & IHC.

Key Applications

Western Blotting
Immunoprecipitation
Immunohistochemistry
Chromatin Immunoprecipitation (ChIP)

Application Notes

Chromatin Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et al. (2005). Human Molecular Genetics. 14(7):953–965.)

Biological Information
Immunogen	His-tagged recombinant protein corresponding to human CTCF.
Epitope	Unknown
Clone	1.2.1.5.4
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes CTCF.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_006556.1
Entrez Gene Summary	This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq].
Gene Symbol	CTCF
Purification Method	Protein G Purified
UniProt Number	P49711
UniProt Summary	FUNCTION: Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.r/>SIZE: 727 amino acids; 82785 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes. SIMILARITY: Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers.
Molecular Weight	~ 130 kDa Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition. The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et al, (2006) Journal of Cell Science 119, 1746-1759) for additional details.

Biological Information

Immunogen

His-tagged recombinant protein corresponding to human CTCF.

Epitope

Unknown

Clone

1.2.1.5.4

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

This antibody recognizes CTCF.

Isotype

IgG1κ

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_006556.1

Entrez Gene Summary

This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq].

Gene Symbol

CTCF

Purification Method

Protein G Purified

UniProt Number

P49711

UniProt Summary

FUNCTION: Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.r/>SIZE: 727 amino acids; 82785 Da

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes.

SIMILARITY: Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers.

Molecular Weight

~ 130 kDa
Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition.
The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et al, (2006) Journal of Cell Science 119, 1746-1759) for additional details.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in K562 cell lysate. Western Blot Analysis: 1 µg/ml of this antibody detected CTCF on 10 µg of K562 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected CTCF on 10 µg of K562 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CTCF, clone 1.2.1.5.4 (mouse monoclonal)

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