Anti-phospho-Neuroligin-1/NL-1 (Thr739) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
M, R	WB, IP	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

M, R

WB, IP

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	ABN1707
Description	Anti-phospho-Neuroligin-1/NL-1 (Thr739)
Alternate Names	Neuroligin-1, Thr739-phosphorylated NL-1, Thr739-phosphorylated
Background Information	Neuroligin-1 (UniProt Q99K10; also known as NL-1) is encoded by the Nlgn1 (also known as Kiaa1070) gene (Gene ID 192167) in murine species. Neuroligins are single-transmembrane postsynaptic proteins that contain a large extracellular acetylcholinesterase-like domain and a cytoplasmic tail (c-tail) with many protein-protein interaction domains. Neuroligin-1 (NL-1) is localized predominantly to excitatory synapses and NL-2 is confined primarily to inhibitory synapses, while NL-3 is found among both types of synapses and NL-4 is enriched at glycinergic synapses. NL-1 is involved in diverse forms of synaptic plasticity across species. NL-1 overexpression enhances evoked excitatory postsynaptic currents (EPSCs) and synaptogenesis, while chronic blockade of NMDA receptors (NMDARs) or CaMKII diminishes NL-1–mediated synaptic potentiation. CaMKII is shown to phosphorylate NL-1 on Thr739 and NL-1 T739A mutation markedly reduces the basal and activity-induced NL-1 surface expression. NL-1 is produced with a signal peptide (a.a. 1-45) that is posttranslationally removed to yield the mature protein with a large extracellular domain (a.a. 46-697), followed by a transmembrane segment (a.a. 698-718) and a cytoplasmic tail (a.a. 719-843).

Description

Catalogue Number

ABN1707

Description

Anti-phospho-Neuroligin-1/NL-1 (Thr739)

Alternate Names

Neuroligin-1, Thr739-phosphorylated
NL-1, Thr739-phosphorylated

Background Information

Neuroligin-1 (UniProt Q99K10; also known as NL-1) is encoded by the Nlgn1 (also known as Kiaa1070) gene (Gene ID 192167) in murine species. Neuroligins are single-transmembrane postsynaptic proteins that contain a large extracellular acetylcholinesterase-like domain and a cytoplasmic tail (c-tail) with many protein-protein interaction domains. Neuroligin-1 (NL-1) is localized predominantly to excitatory synapses and NL-2 is confined primarily to inhibitory synapses, while NL-3 is found among both types of synapses and NL-4 is enriched at glycinergic synapses. NL-1 is involved in diverse forms of synaptic plasticity across species. NL-1 overexpression enhances evoked excitatory postsynaptic currents (EPSCs) and synaptogenesis, while chronic blockade of NMDA receptors (NMDARs) or CaMKII diminishes NL-1–mediated synaptic potentiation. CaMKII is shown to phosphorylate NL-1 on Thr739 and NL-1 T739A mutation markedly reduces the basal and activity-induced NL-1 surface expression. NL-1 is produced with a signal peptide (a.a. 1-45) that is posttranslationally removed to yield the mature protein with a large extracellular domain (a.a. 46-697), followed by a transmembrane segment (a.a. 698-718) and a cytoplasmic tail (a.a. 719-843).

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.

Product Information

Format

Affinity Purified

Presentation

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.

Applications
Application	Anti-Neuroligin Antibody is an antibody against phospho-Neuroligin-1/NL-1 (Thr739) for use in Western Blotting, Immunoprecipitation.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Western Blotting Analysis: A representative lot detected CaMKII-catalyzed Thr739 phosphorylation of mouse NL-1 C-terminal fragment GST fusion in a kinase assay (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64). Western Blotting Analysis: A representative lot specifically detected the target phospho-band in lysates from transfected cells co-expressing constitutively active CaMKII (T286D) and wild-type, but not T739A mutan, NL-1 (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64). Western Blotting Analysis: A representative lot detected an enhanced NL-1 Thr730 phosphorylation in cultured embryonic rat cortical neurons upon GABA(A) receptor antagonist BCC (Cat. No. 505875) treatment. Co-treatment with NMDAR and AMPAR antagonists blocked the effect seen with BCC treatment alone (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64). Immunoprecipitation Analysis: A representative lot immunoprecipitated a ~120 kDa Thr739-phosphorylated NL-1 from wild-type, but not Nlgn1-knockout, mouse brain tissue lysates (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64). Immunoprecipitation Analysis: A representative lot immunoprecipitated less Thr739-phosphorylated NL-1 from visual cortex of postnatal mice following a 5-day dark-rearing (DR) period (no light from P21-P26). A two-hour light exposure after the 5-day DR period restored visual cortex NL-1 phosphorylation the control level (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64).

Applications

Application

Anti-Neuroligin Antibody is an antibody against phospho-Neuroligin-1/NL-1 (Thr739) for use in Western Blotting, Immunoprecipitation.

Key Applications

Western Blotting
Immunoprecipitation

Application Notes

Western Blotting Analysis: A representative lot detected CaMKII-catalyzed Thr739 phosphorylation of mouse NL-1 C-terminal fragment GST fusion in a kinase assay (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64).
Western Blotting Analysis: A representative lot specifically detected the target phospho-band in lysates from transfected cells co-expressing constitutively active CaMKII (T286D) and wild-type, but not T739A mutan, NL-1 (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64).
Western Blotting Analysis: A representative lot detected an enhanced NL-1 Thr730 phosphorylation in cultured embryonic rat cortical neurons upon GABA(A) receptor antagonist BCC (Cat. No. 505875) treatment. Co-treatment with NMDAR and AMPAR antagonists blocked the effect seen with BCC treatment alone (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64).
Immunoprecipitation Analysis: A representative lot immunoprecipitated a ~120 kDa Thr739-phosphorylated NL-1 from wild-type, but not Nlgn1-knockout, mouse brain tissue lysates (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64).
Immunoprecipitation Analysis: A representative lot immunoprecipitated less Thr739-phosphorylated NL-1 from visual cortex of postnatal mice following a 5-day dark-rearing (DR) period (no light from P21-P26). A two-hour light exposure after the 5-day DR period restored visual cortex NL-1 phosphorylation the control level (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64).

Biological Information
Immunogen	Linear peptide corresponding to a mouse Neuroligin-1 sequence containing phosphorylated Thr739.
Epitope	pThr739.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	This polyclonal antibody specifically detected the target phospho-band in lysates from transfected cells co-expressing constitutively active CaMKII (T286D) and wild-type, but not T739A mutan, NL-1. The Thr739 designation is based on murine & rat NL-1 sequence (UniProt Q99K10 & Q62765). The equivalent site in human NL-1 is Thr736 (UniProt Q8N2Q7). The target phosphorylation site is present in both human spliced isoforms (Thr719 of isoform 2) and all four rat spliced isoforms (Thr710/730/719 of isoform 2/3/4), but is missing in mouse spliced isoform 2.
Species Reactivity	Mouse Rat
Species Reactivity Note	Mouse, Rat. Predicted to react with Human, Bovine, Chicken, Rhesus Macaque based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_619607
Gene Symbol	Nlgn1 Kiaa1070
Modifications	Phosphorylation
Purification Method	Affinity purfied.
UniProt Number	Q99K10
Molecular Weight	88.93/87.09 kDa (human isoform 1/2), 89.12 kDa (mouse isoform 1), and 89.26/85.96/88.23/87.00 kDa (rat isoform 1/2/3/4) calculated (Signal peptide a.a. 1-45 removed). ~120 kDa reported (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64). Uncharacterized band(s) may appear in some lysates.

Biological Information

Immunogen

Linear peptide corresponding to a mouse Neuroligin-1 sequence containing phosphorylated Thr739.

Epitope

pThr739.

Concentration

Please refer to lot specific datasheet.

Host

Rabbit

Specificity

This polyclonal antibody specifically detected the target phospho-band in lysates from transfected cells co-expressing constitutively active CaMKII (T286D) and wild-type, but not T739A mutan, NL-1. The Thr739 designation is based on murine & rat NL-1 sequence (UniProt Q99K10 & Q62765). The equivalent site in human NL-1 is Thr736 (UniProt Q8N2Q7). The target phosphorylation site is present in both human spliced isoforms (Thr719 of isoform 2) and all four rat spliced isoforms (Thr710/730/719 of isoform 2/3/4), but is missing in mouse spliced isoform 2.

Species Reactivity

Mouse
Rat

Species Reactivity Note

Mouse, Rat. Predicted to react with Human, Bovine, Chicken, Rhesus Macaque based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_619607

Gene Symbol

Nlgn1
Kiaa1070

Modifications

Phosphorylation

Purification Method

Affinity purfied.

UniProt Number

Q99K10

Molecular Weight

88.93/87.09 kDa (human isoform 1/2), 89.12 kDa (mouse isoform 1), and 89.26/85.96/88.23/87.00 kDa (rat isoform 1/2/3/4) calculated (Signal peptide a.a. 1-45 removed). ~120 kDa reported (Bemben, M.A., et al. (2014). Nat. Neurosci. 17(1):56-64). Uncharacterized band(s) may appear in some lysates.

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting of CaMKII-catalyzed phosphorylation of mouse NL-1 GST fusion. Western Blotting Analysis: 0.2 µg/mL of this antibody detected CaMKII-catalyzed Thr739 phosphorylation of 6 µg mouse NL-1 C-terminal fragment GST fusion in a kinase assay.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blotting of CaMKII-catalyzed phosphorylation of mouse NL-1 GST fusion.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected CaMKII-catalyzed Thr739 phosphorylation of 6 µg mouse NL-1 C-terminal fragment GST fusion in a kinase assay.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Packaging Information

Material Size

100 μg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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Anti-phospho-Neuroligin-1/NL-1 (Thr739) (rabbit polyclonal)

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