Anti-MAP Kinase 1/2 (Erk1/2),CT, Alexa Fluor® 555 conj.

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Anti-MAP Kinase 1/2 (Erk1/2),CT, Alexa Fluor® 555 conj.

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货号：16-284

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
Ch, H, M, R	FC, ICC, IF, WB	Rb	AlexaFluor®555	Polyclonal Antibody

Description
Catalogue Number	16-284
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-MAP Kinase 1/2 (Erk1/2) Antibody, CT, Alexa Fluor® 555 conjugate
Alternate Names	MAPK1/2 Extracellular signaling related kinase 1/2

References

Product Information
Format	AlexaFluor®555

Applications
Application	This Anti-MAP Kinase 1/2 (Erk1/2) Antibody, CT, Alexa Fluor 555 conjugate is validated for use in FC, IC, IF, WB for the detection of MAP Kinase 1/2 (Erk1/2).
Key Applications	Flow Cytometry Immunocytochemistry Immunofluorescence Western Blotting

Biological Information
Immunogen	38 residue synthetic peptide (CGG-PFTFDMELDDLPKERLKELIFQETARFQPGAPEAP) corresponding to the C-terminal 35 amino acids of the rat 44kDa MAP Kinase 2/Erk2 coupled to KLH. The immunizing sequence is totally conserved in Chinese hamster, is 34/35 identical in mouse, and 32/35 identical in human.
Host	Rabbit
Specificity	Recognizes MAPK1/Erk1, Mr 44kDa, and MAPK2/Erk2.
Isotype	IgG
Species Reactivity	Chicken Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002745.4 NM_138957.2
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol	MAPK1 p40 PRKM2 MAPK2 PRKM1 p42-MAPK p38 p41 ERK ERK-2 ERT1 P42MAPK p41mapk ERK2
Purification Method	ImmunoAffinity Purified
UniProt Number	P28482
UniProt Summary	FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). COFACTOR: Magnesium (By similarity). SIZE: 360 amino acids; 41390 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4. DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight	42 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by immunocytochemistry and Flow Cytometry.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Product Usage Statements

Quality Assurance

Routinely evaluated by immunocytochemistry and Flow Cytometry.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Storage and Shipping Information
Storage Conditions	1 year at 4°C from date of shipment

Packaging Information
Material Size	50 µg

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Anti-MAP Kinase 1/2 (Erk1/2),CT, Alexa Fluor® 555 conj.

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