Anti-FOXO3a/FKHRL1 (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

Purified

Polyclonal Antibody

Description
Catalogue Number	07-702
Replaces	04-1007
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-FOXO3a/FKHRL1 Antibody
Alternate Names	AF6q21 protein Forkhead in rhabdomyosarcoma-like 1 forkhead box O3 forkhead box O3A forkhead homolog (rhabdomyosarcoma) like 1 forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
Background Information	The forkhead family of transcription factors is a large group of proteins that share a common conserved 100 amino acid DNA binding domain, which is termed a winged-helix or forkhead domain after the founding member of this group, the forkhead gene in Drosophila. In addition to their roles during normal development, recent studies have demonstrated that members of the forkhead family may participate in several kinds of neoplasia. In particular, 3 genes of the human forkhead family, FKHR, FKHRL1, and AFX, which are orthologues of DAF-16 in Caenorhabditis elegans, were identified at the chromosomal breakpoints in human neoplasms. These three genes have been assigned to the FOXO (named for “Forkhead Box, group O”) subfamily of forkhead transcription factors. The forkhead factors, including FKHRL1, regulate their activation through phosphorylation by proto-oncogene Akt (protein kinase B) and/or SGK (serum- and glucocorticoid- induced kinases) in mammalian cells and the phosphorylated form localized at the cytoplasm is recognized to be an inactivated form, whereas nuclear-imported dephosphorylated FKHRL1 is functional as an active transcriptional factor.

Description

Catalogue Number

07-702

Replaces

04-1007

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-FOXO3a/FKHRL1 Antibody

Alternate Names

AF6q21 protein
Forkhead in rhabdomyosarcoma-like 1
forkhead box O3
forkhead box O3A
forkhead homolog (rhabdomyosarcoma) like 1
forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1

Background Information

The forkhead family of transcription factors is a large group of proteins that share a common conserved 100 amino acid DNA binding domain, which is termed a winged-helix or forkhead domain after the founding member of this group, the forkhead gene in Drosophila. In addition to their roles during normal development, recent studies have demonstrated that members of the forkhead family may participate in several kinds of neoplasia. In particular, 3 genes of the human forkhead family, FKHR, FKHRL1, and AFX, which are orthologues of DAF-16 in Caenorhabditis elegans, were identified at the chromosomal breakpoints in human neoplasms. These three genes have been assigned to the FOXO (named for “Forkhead Box, group O”) subfamily of forkhead transcription factors. The forkhead factors, including FKHRL1, regulate their activation through phosphorylation by proto-oncogene Akt (protein kinase B) and/or SGK (serum- and glucocorticoid- induced kinases) in mammalian cells and the phosphorylated form localized at the cytoplasm is recognized to be an inactivated form, whereas nuclear-imported dephosphorylated FKHRL1 is functional as an active transcriptional factor.

Product Information
Format	Purified
Control	3T3/A31 Cell Lysate.
Presentation	Antigen affinity purified rabbit polyclonal, followed by anti-GST depletion, in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.

Product Information

Format

Purified

Control

3T3/A31 Cell Lysate.

Presentation

Antigen affinity purified rabbit polyclonal, followed by anti-GST depletion, in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.

Applications
Application	Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A.
Key Applications	Western Blotting

Applications

Application

Use Anti-FOXO3a/FKHRL1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect FOXO3a/FKHRL1 also known as AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, forkhead box O3A.

Key Applications

Western Blotting

Biological Information
Immunogen	GST fusion protein corresponding to residues 330-673 of human FKHRL1/FOXO3A.
Epitope	a.a. 330-673
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes FKHRL1/FOXO3A, Mr 90-95 kDa.
Isotype	IgG
Species Reactivity	Human Mouse
Species Reactivity Note	Proven to react with human and mouse. Not tested on other species.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001455
Entrez Gene Summary	This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Gene Symbol	FOXO3 FKHRL1 FOXO2 MGC12739 FKHRL1P2 AF6q21 MGC31925 FOXO3A DKFZp781A0677
Purification Method	Affinity Purfied
UniProt Number	O43524
UniProt Summary	FUNCTION: SwissProt: O43524 # Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. SIZE: 673 amino acids; 71277 Da SUBUNIT: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. SUBCELLULAR LOCATION: Cytoplasm, cytosol. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors. TISSUE SPECIFICITY: Ubiquitous. PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation.DISEASE:SwissProt: O43524 # A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. SIMILARITY: SwissProt: O43524 ## Contains 1 fork-head DNA-binding domain.
Molecular Weight	Approx. 90-95 kDa

Biological Information

Immunogen

GST fusion protein corresponding to residues 330-673 of human FKHRL1/FOXO3A.

Epitope

a.a. 330-673

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes FKHRL1/FOXO3A, Mr 90-95 kDa.

Isotype

IgG

Species Reactivity

Human
Mouse

Species Reactivity Note

Proven to react with human and mouse. Not tested on other species.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_001455

Entrez Gene Summary

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Gene Symbol

FOXO3
FKHRL1
FOXO2
MGC12739
FKHRL1P2
AF6q21
MGC31925
FOXO3A
DKFZp781A0677

Purification Method

Affinity Purfied

UniProt Number

O43524

UniProt Summary

FUNCTION: SwissProt: O43524 # Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
SIZE: 673 amino acids; 71277 Da
SUBUNIT: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation.
SUBCELLULAR LOCATION: Cytoplasm, cytosol. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
TISSUE SPECIFICITY: Ubiquitous.
PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation.DISEASE:SwissProt: O43524 # A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
SIMILARITY: SwissProt: O43524 ## Contains 1 fork-head DNA-binding domain.

Molecular Weight

Approx. 90-95 kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on 3T3/A31 cell lysates. Western Blot Analysis: A 0.5 g/mL dilution of this antibody was used to detect FOXO3a/FKHRL1 in 3T3/A31 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by Western Blot on 3T3/A31 cell lysates.

Western Blot Analysis:
A 0.5 g/mL dilution of this antibody was used to detect FOXO3a/FKHRL1 in 3T3/A31 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-FOXO3a/FKHRL1 (rabbit polyclonal)

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