Anti-phospho-SMC1 (Ser957), Clone 5D11G5

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M

Purified

Monoclonal Antibody

Description
Catalogue Number	05-786
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5
Overview	SMC1 is a ATPase and subunit of the multiprotein cohesin complex, a protein involved in chromosome segregation and in double-strand DNA break (DSB) repair. Serine 957 is phosphorylated by ATM in response to DSBs and is involved in the S-phase DNA damage checkpoint.

Description

Catalogue Number

05-786

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5

Overview

SMC1 is a ATPase and subunit of the multiprotein cohesin complex, a protein involved in chromosome segregation and in double-strand DNA break (DSB) repair. Serine 957 is phosphorylated by ATM in response to DSBs and is involved in the S-phase DNA damage checkpoint.

Product Information
Format	Purified
Presentation	0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Product Information

Format

Purified

Presentation

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Applications
Application	Detect phospho-SMC1 (Ser957) with Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 (Mouse Monoclonal Antibody), that has been shown to work in WB.
Key Applications	Western Blotting

Applications

Application

Detect phospho-SMC1 (Ser957) with Anti-phospho-SMC1 (Ser957) Antibody, clone 5D11G5 (Mouse Monoclonal Antibody), that has been shown to work in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 951-962 (SQEEGS[pS]QGEDS) of human SMC1
Clone	5D11G5
Host	Mouse
Specificity	phospho-SMC1 (Ser957)
Isotype	IgG1
Species Reactivity	Human Mouse
Species Reactivity Note	Predicted to cross-react with xenopus and bovine based on sequence homology
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_006306
Entrez Gene Summary	Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Gene Symbol	SMC1A SMCB SMC1alpha DXS423E DKFZp686L19178 SMC1 SMC1L1 Smcb OTTHUMP00000061876 KIAA0178 SB1.8 MGC138332 CDLS2 Sb1.8
Modifications	Phosphorylation
Purification Method	Protein G Purified
UniProt Number	Q14683
UniProt Summary	FUNCTION: SwissProt: Q14683 # Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. SIZE: 1233 amino acids; 143233 Da SUBUNIT: Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesin complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with KNTC2. SUBCELLULAR LOCATION: Nucleus. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis. DOMAIN: SwissProt: Q14683 The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V- shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity). PTM: Phosphorylated by ATM upon ionizing radiation in a NBS1- dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. DISEASE: SwissProt: Q14683 # Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. SIMILARITY: Belongs to the SMC family. SMC1 subfamily.
Molecular Weight	Mr 150kDa

Biological Information

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 951-962 (SQEEGS[pS]QGEDS) of human SMC1

Clone

5D11G5

Host

Mouse

Specificity

phospho-SMC1 (Ser957)

Isotype

IgG1

Species Reactivity

Human
Mouse

Species Reactivity Note

Predicted to cross-react with xenopus and bovine based on sequence homology

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_006306

Entrez Gene Summary

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.

Gene Symbol

SMC1A
SMCB
SMC1alpha
DXS423E
DKFZp686L19178
SMC1
SMC1L1
Smcb
OTTHUMP00000061876
KIAA0178
SB1.8
MGC138332
CDLS2
Sb1.8

Modifications

Phosphorylation

Purification Method

Protein G Purified

UniProt Number

Q14683

UniProt Summary

FUNCTION: SwissProt: Q14683 # Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
SIZE: 1233 amino acids; 143233 Da
SUBUNIT: Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesin complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with KNTC2.
SUBCELLULAR LOCATION: Nucleus. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
DOMAIN: SwissProt: Q14683 The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V- shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure (By similarity).
PTM: Phosphorylated by ATM upon ionizing radiation in a NBS1- dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
DISEASE: SwissProt: Q14683 # Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
SIMILARITY: Belongs to the SMC family. SMC1 subfamily.

Molecular Weight

Mr 150kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoblot on RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoblot on RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C

Storage and Shipping Information

Storage Conditions

2 years at -20°C

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-phospho-SMC1 (Ser957), Clone 5D11G5

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