Anti-PI3 Kinase, p110a (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB, IP, IHC, ELISA	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, R

WB, IP, IHC, ELISA

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-481
Replaces	04-399
Description	Anti-PI3 Kinase Antibody, p110α
Alternate Names	PI3-kinase p110 subunit alpha PtdIns-3-kinase p110 phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha phosphatidylinositol 3-kinase, catalytic, alpha polypeptide phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform phosphoinositide-3-kinase, catalytic, alpha polypeptide
Background Information	Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class I p110 catalytic domains. Class I(A) include α, β, γ and class I(B). They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.

Description

Catalogue Number

09-481

Replaces

04-399

Description

Anti-PI3 Kinase Antibody, p110α

Alternate Names

PI3-kinase p110 subunit alpha
PtdIns-3-kinase p110
phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
phosphoinositide-3-kinase, catalytic, alpha polypeptide

Background Information

Phosphoinositol-3-kinases (PI3 Kinase, PI3K) are a family of lipid kinases that mediate many intracellular signaling responses in both physiological and pathophysiological states. PI3 Kinase is a heteroduplex with three classes (I, II, & III) of catalytic domain. The class I PI3 Kinases are made up of a p85 regulatory protein and a p110 catalytic domain. There are 4 isoforms of the class I p110 catalytic domains. Class I(A) include α, β, γ and class I(B). They are activated downstream via receptor tyrosine kinases or G-protein coupled receptors. Once activated, PI3K generates phosphatidylinositol 3,4,5-trisphosphate (PIP3) from PIP2. It was later found that PTEN, a lipid phosphatase, acts opposite to PI3K by dephosphorylating PIP3, converting it to PIP2, thus de-activating the effects of PI3K. Increased levels of PIP3 in the cell leads to the activation of many key signaling regulators including Akt and PDK as well as calcium release. One of the primary roles of PI3K is its regulation of cell growth and cell metabolism. Both of these play a critical role in cancer as some believe that a tumor’s shift to glycolysis is due to the activation of PI3K. PI3 kinases have been linked with numerous disease states, including cancer, diabetes, allergic response, hypertension, cardiac contractility, atherosclerosis, sepsis and autoimmune/ inflammatory disorders.

Product Information
Format	Affinity Purified
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Product Information

Format

Affinity Purified

Presentation

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Applications
Application	Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110α validated for use in WB, IP, IH, ELISA.
Key Applications	Western Blotting Immunoprecipitation Immunohistochemistry ELISA

Applications

Application

Detect PI3 Kinase using this Anti-PI3 Kinase Antibody, p110α validated for use in WB, IP, IH, ELISA.

Key Applications

Western Blotting
Immunoprecipitation
Immunohistochemistry
ELISA

Biological Information
Immunogen	KLH-conjugated synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform.
Epitope	Internal
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	Recognizes PI3 Kinase p110α.
Species Reactivity	Human Rat
Species Reactivity Note	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_006209.2
Entrez Gene Summary	Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq].
Gene Symbol	EC 2.7.1.153 MGC142161 MGC142163 PI3K p110-alpha
Purification Method	Immunogen Affinity Purified
UniProt Number	P42336
UniProt Summary	FUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate. SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 By similarity. INVOLVEMENT IN DISEASE: Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. Defects in PIK3CA are associated with breast cancer [MIM:114480]. Defects in PIK3CA are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550]. Ref.14 PI3KCA mutations affecting exons 9 and 20 display gender- and tissue-specific patterns, thus suggesting that the different amino acid changes could exert distinct functional effects on the oncogenic properties of this enzyme. Furthermore, sexual dimorphisms and tissue specific factors might directly or indirectly influence the occurrence of PI3KCA cancer alleles. SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family. Contains 1 C2 domain. Contains 1 PI3K/PI4K domain.
Molecular Weight	110 kDa

Biological Information

Immunogen

KLH-conjugated synthetic linear peptide corresponding to an internal region of class I catalytic domain of PI3 Kinase that is specific to the p110 alpha isoform.

Epitope

Internal

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

Recognizes PI3 Kinase p110α.

Species Reactivity

Human
Rat

Species Reactivity Note

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_006209.2

Entrez Gene Summary

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq].

Gene Symbol

EC 2.7.1.153
MGC142161
MGC142163
PI3K
p110-alpha

Purification Method

Immunogen Affinity Purified

UniProt Number

P42336

UniProt Summary

FUNCTION: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.

CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.

SUBUNIT STRUCTURE: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 By similarity.

INVOLVEMENT IN DISEASE: Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].
Defects in PIK3CA are associated with breast cancer [MIM:114480].
Defects in PIK3CA are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.
Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550]. Ref.14
PI3KCA mutations affecting exons 9 and 20 display gender- and tissue-specific patterns, thus suggesting that the different amino acid changes could exert distinct functional effects on the oncogenic properties of this enzyme. Furthermore, sexual dimorphisms and tissue specific factors might directly or indirectly influence the occurrence of PI3KCA cancer alleles.

SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.
Contains 1 C2 domain.
Contains 1 PI3K/PI4K domain.

Molecular Weight

110 kDa

Product Usage Statements
Quality Assurance	Evaluated by western blot on Jurkat cell extract. Western Blotting Analysis: 0.5 µg/mL (1:2,000) dilution of this antibody detected PI3K, p110α in Jurkat cell extract.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by western blot on Jurkat cell extract.

Western Blotting Analysis: 0.5 µg/mL (1:2,000) dilution of this antibody detected PI3K, p110α in Jurkat cell extract.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8ºC from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8ºC from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-PI3 Kinase, p110a (rabbit polyclonal)

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