Anti-a-Tubulin, clone DM1A

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

Qiagen 凯杰 Biosharp Omega

客服热线 0771-3293894

首页抗体Millipore 密理博>

图片仅供参考，请以实物为准

Anti-a-Tubulin, clone DM1A

市场价：: 0.00

促销价：: ¥0.00

货号：05-829

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M, Po, B, Av, Gp, H	ICC, IF, WB	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-829
Replaces	04-1117
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-α-Tubulin Antibody, clone DM1A
Alternate Names	Alpha-tubulin 3 Tubulin B-alpha-1 Tubulin alpha-3 chain tubulin, alpha 1a tubulin, alpha 3 tubulin, alpha, brain-specific
Background Information	Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

References

Product Information
Format	Purified
Control	A431 cell lysate.
Presentation	Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol.

Applications
Application	Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.
Key Applications	Immunocytochemistry Immunofluorescence Western Blotting
Application Notes	Immunofluorescence: (Breitling, F., 1986)

Biological Information
Immunogen	Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).
Epitope	a.a. 426-450
Clone	DM1A
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.
Isotype	IgG1
Species Reactivity	Rat Mouse Pig Bovine Avian Guinea Pig Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_006000
Entrez Gene Summary	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
Gene Symbol	TUBA1A hum-a-tub2 FLJ25113 B-ALPHA-1 TUBA3 hum-a-tub1
Purification Method	Protein G Purified
UniProt Number	Q71U36
UniProt Summary	FUNCTION: SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. SIZE: 451 amino acids; 50136 Da SUBUNIT: Dimer of alpha and beta chains. PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity). SIMILARITY: SwissProt: Q71U36 ## Belongs to the tubulin family.
Molecular Weight	50-60 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by western blot on non-stimulated A431 lysate. Western Blot Analysis: 0.1-1 µg/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.