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Anti-Properdin (rabbit polyclonal)

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货号：ABF185

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number ABF185
Description Anti-Properdin Antibody
Alternate Names
  • Complement factor P
  • Properdin
Background Information Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.
References
Product Information
Format Affinity Purified
Presentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-Properdin Antibody is validated for use in Western Blotting for the detection of Properdin.
Key Applications
  • Western Blotting
Application Notes Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Properdin in 10 µg of mouse serum.
Biological Information
Immunogen KLH-conjugated linear peptide corresponding to a TSP type-1 domain 3 sequence of human Properdin.
Epitope Within TSP type-1 domain 3.
Concentration Please refer to lot specific datasheet.
Host Rabbit
Specificity This polyclonal antibody detects properdin by targeting a sequence in the fourth TSP type-1 domain (TSP type-1 domain 3).
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Human, Mouse, Rat. Predicted to react with Non-Human Primate based on 100% sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CFP
  • PFC
  • PFD
Purification Method Affinity purified.
UniProt Number
Molecular Weight ~52 kDa observed. 48.49/47.94/48.18 kDa (human/mouse/rat mature form) and 51.28/50.33/50.57 kDa (human/mouse/rat pro-form) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Properdin in 10 µg of rat serum.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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