Anti-CUG-BP1, clone 3B1 (mouse monoclonal IgG)

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, M, Po, R, Rb	EMSA, IP, WB, ICC	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

B, H, M, Po, R, Rb

EMSA, IP, WB, ICC

Purified

Monoclonal Antibody

Description
Catalogue Number	05-621
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-CUGBP1 Antibody, clone 3B1
Background Information	Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3'-UTR.

Description

Catalogue Number

05-621

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-CUGBP1 Antibody, clone 3B1

Background Information

Myotonic dystrophy (MD) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase (Mt-PK) gene. A (CUG)n oligonucleotides triplet repeat pre-mRNA/mRNA binding protein may play an important role in DM pathogenesis. HeLa cell protein, CUG-BP1, has been purified based upon its ability to bind specifically to (CUG)8 oligonucleotides in vitro. CUG-BP1 is the major (CUG)8 binding activity in normal cells. CUG-BP1 has been identified as isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. The CUG-BP/hNab50 protein is localized predominantly in the nucleus and is associated with polyadenylated RNAs in vivo. In vitro RNA-binding/photocrosslinking studies demonstrate that CUG-BP/hNab50 binds to RNAs containing the Mt-PK 3'-UTR.

Product Information
Format	Purified
Control	HeLa lysates
Presentation	0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Product Information

Format

Purified

Control

HeLa lysates

Presentation

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Applications
Application	Anti-CUGBP1 Antibody, clone 3B1 is a high quality Mouse Monoclonal Antibody for the detection of CUGBP1 & has been validated in EMSA, IP, WB, ICC.
Key Applications	Electrophoretic Mobility Shift Assay Immunoprecipitation Western Blotting Immunocytochemistry

Applications

Application

Anti-CUGBP1 Antibody, clone 3B1 is a high quality Mouse Monoclonal Antibody for the detection of CUGBP1 & has been validated in EMSA, IP, WB, ICC.

Key Applications

Electrophoretic Mobility Shift Assay
Immunoprecipitation
Western Blotting
Immunocytochemistry

Biological Information
Immunogen	Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50
Clone	clone 3B1
Host	Mouse
Specificity	CUG-BP1
Isotype	IgG
Species Reactivity	Bovine Human Mouse Pig Rat Rabbit
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_006560 and others
Entrez Gene Summary	Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene Symbol	CUGBP1 BRUNOL2 CELF-1 CUGBP NAB50 CELF1 CUG-BP hNab50 CUG-BP1
Purification Method	Protein G Chromatography
UniProt Number	Q92879
UniProt Summary	FUNCTION: SwissProt: Q92879 # RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver (By similarity). SIZE: 486 amino acids; 52063 Da SUBUNIT: Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Associates with polysomes (By similarity). Interacts with PARN. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=RNA-binding activity is detected in both nuclear and cytoplasmic compartments. TISSUE SPECIFICITY: Ubiquitous. PTM: Phosphorylated. Its phosphorylation status increases in senescent cells. DISEASE:SwissProt: Q92879 # CUGBP1 may be involved in the mechanism of myotonic distrophy. It binds to the CUG repeat expansion of the CC untranslated region of the myotonin protein kinase (Mt-PK) gene. SIMILARITY: SwissProt: Q92879 ## Belongs to the CELF/BRUNOL family. & Contains 3 RRM (RNA recognition motif) domains.
Molecular Weight	50kDa

Biological Information

Immunogen

Full-length GST fusion protein corresponding to human CUG-BP1, also known as heterogeneous nuclear ribonucleoprotein (hnRNP) hNab50

Clone

clone 3B1

Host

Mouse

Specificity

CUG-BP1

Isotype

IgG

Species Reactivity

Bovine
Human
Mouse
Pig
Rat
Rabbit

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_006560 and others

Entrez Gene Summary

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Symbol

CUGBP1
BRUNOL2
CELF-1
CUGBP
NAB50
CELF1
CUG-BP
hNab50
CUG-BP1

Purification Method

Protein G Chromatography

UniProt Number

Q92879

UniProt Summary

FUNCTION: SwissProt: Q92879 # RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver (By similarity).

SIZE: 486 amino acids; 52063 Da

SUBUNIT: Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Associates with polysomes (By similarity). Interacts with PARN.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
TISSUE SPECIFICITY: Ubiquitous.

PTM: Phosphorylated. Its phosphorylation status increases in senescent cells.
DISEASE:SwissProt: Q92879 # CUGBP1 may be involved in the mechanism of myotonic distrophy. It binds to the CUG repeat expansion of the CC untranslated region of the myotonin protein kinase (Mt-PK) gene.

SIMILARITY: SwissProt: Q92879 ## Belongs to the CELF/BRUNOL family. & Contains 3 RRM (RNA recognition motif) domains.

Molecular Weight

50kDa

Product Usage Statements
Quality Assurance	routinely evaluated by immunoblot on HeLa nuclear extract
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

routinely evaluated by immunoblot on HeLa nuclear extract

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C

Storage and Shipping Information

Storage Conditions

2 years at -20°C

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-CUG-BP1, clone 3B1 (mouse monoclonal IgG)

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