Anti-Notch 1, extracellular domain (rabbit polyclonal IgG)

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, M	WB	Rb	Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

R, M

Purified

Polyclonal Antibody

Description
Catalogue Number	07-218
Replaces	04-1046
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Notch1 Antibody, extracellular domain
Background Information	The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems. This family of proteins function both as membrane cell receptors and as transcription factors. Activation of Notch by cell-cell interactions causes a transcription inhibitory effect that enables inhibition of differentiation in some cells but not in others. As a consequence, some cells adopt a particular fate while other progenitors remain uncommitted. The Notch protein is important in cell fate during myogenesis, neurogenesis, oogenesis, and wing and eye development in Drosophila. In mammals, four Notch genes were identified (Notch 1-4) that are expressed in a wide variety of cells and play a crucial role in differentiation and development.

Description

Catalogue Number

07-218

Replaces

04-1046

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-Notch1 Antibody, extracellular domain

Background Information

The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems. This family of proteins function both as membrane cell receptors and as transcription factors. Activation of Notch by cell-cell interactions causes a transcription inhibitory effect that enables inhibition of differentiation in some cells but not in others. As a consequence, some cells adopt a particular fate while other progenitors remain uncommitted. The Notch protein is important in cell fate during myogenesis, neurogenesis, oogenesis, and wing and eye development in Drosophila. In mammals, four Notch genes were identified (Notch 1-4) that are expressed in a wide variety of cells and play a crucial role in differentiation and development.

Product Information
Format	Purified
Control	L/N17 transfected Notch1 cell lysate
Presentation	Protein A purified immunoglobulin presented in 0.1M Tris-Glycine, 0.15M NaCl, and 0.05% Sodium Azide, pH 7.4

Product Information

Format

Purified

Control

L/N17 transfected Notch1 cell lysate

Presentation

Protein A purified immunoglobulin presented in 0.1M Tris-Glycine, 0.15M NaCl, and 0.05% Sodium Azide, pH 7.4

Applications
Application	Anti-Notch1 Antibody, extracellular domain is a high quality Rabbit Polyclonal Antibody for the detection of Notch1 & has been validated in WB.
Key Applications	Western Blotting

Applications

Application

Anti-Notch1 Antibody, extracellular domain is a high quality Rabbit Polyclonal Antibody for the detection of Notch1 & has been validated in WB.

Key Applications

Western Blotting

Biological Information
Immunogen	N-terminal GST fusion protein corresponding to residues 381-853 of rat Notch1
Host	Rabbit
Specificity	Notch1
Isotype	IgG
Species Reactivity	Rat Mouse
Species Reactivity Note	Used in mouse in the following publication: Gulino, R., et al, European Journal of Neuroscience, Vol. 31, pp. 1423–1434, 2010.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_017617
Entrez Gene Summary	This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
Gene Symbol	NOTCH1 notch1 hN1 TAN1
Purification Method	Protein A chromatography
UniProt Number	P46531
UniProt Summary	FUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity). SIZE: 2556 amino acids; 272500 Da SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). & Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity). TISSUE SPECIFICITY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity). DISEASE: SwissProt: P46531 # NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. & Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population. SIMILARITY: SwissProt: P46531 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 36 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.
Molecular Weight	260kDa

Biological Information

Immunogen

N-terminal GST fusion protein corresponding to residues 381-853 of rat Notch1

Host

Rabbit

Specificity

Notch1

Isotype

IgG

Species Reactivity

Rat
Mouse

Species Reactivity Note

Used in mouse in the following publication: Gulino, R., et al, European Journal of Neuroscience, Vol. 31, pp. 1423–1434, 2010.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_017617

Entrez Gene Summary

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.

Gene Symbol

NOTCH1
notch1
hN1
TAN1

Purification Method

Protein A chromatography

UniProt Number

P46531

UniProt Summary

FUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).
SIZE: 2556 amino acids; 272500 Da
SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). & Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity).
TISSUE SPECIFICITY: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
DISEASE: SwissProt: P46531 # NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. & Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population.
SIMILARITY: SwissProt: P46531 ## Belongs to the NOTCH family. & Contains 5 ANK repeats. & Contains 36 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.

Molecular Weight

260kDa

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot on RIPA lysates from L/N17 rat Notch1 transfected fibroblast cells
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Routinely evaluated by immunoblot on RIPA lysates from L/N17 rat Notch1 transfected fibroblast cells

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-Notch 1, extracellular domain (rabbit polyclonal IgG)

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