Anti-CAR, clone RmcB

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, WB	M	Purified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

ICC, WB

Purified

Monoclonal Antibody

Description
Catalogue Number	05-644
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-CAR Antibody, clone RmcB
Alternate Names	46 kD coxsackievirus and adenovirus receptor (CAR) protein CVB3 binding protein CVB3-binding protein Coxsackievirus B-adenovirus receptor coxsackie virus B receptor coxsackie virus and adenovirus receptor
Background Information	The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.

Description

Catalogue Number

05-644

Brand Family

Upstate

Trade Name

Upstate

Description

Anti-CAR Antibody, clone RmcB

Alternate Names

46 kD coxsackievirus and adenovirus receptor (CAR) protein
CVB3 binding protein
CVB3-binding protein
Coxsackievirus B-adenovirus receptor
coxsackie virus B receptor
coxsackie virus and adenovirus receptor

Background Information

The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.

Product Information
Format	Purified
Control	Lung tissue
Presentation	Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.

Product Information

Format

Purified

Control

Lung tissue

Presentation

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.

Applications
Application	Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.
Key Applications	Immunocytochemistry Western Blotting
Application Notes	Immunocytochemistry: 1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.

Applications

Application

Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.

Key Applications

Immunocytochemistry
Western Blotting

Application Notes

Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.

Biological Information
Immunogen	Native human coxsackie virus-adenovirus receptor protein.
Clone	RmcB
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	Recognizes CAR at MW ~46 kDa.
Isotype	IgG1
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_001338
Entrez Gene Summary	The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.
Gene Symbol	CXADR CAR hCAR HCVADR HCAR
Purification Method	Protein G Purified
UniProt Number	P78310
UniProt Summary	FUNCTION: SwissProt: P78310 # Receptor for group B coxsackieviruses and subgroup C of adenoviruses (AD2 and AD5), susceptibility to infection is correlated to its level of expression and availability at the plasma membrane of cells. SIZE: 365 amino acids; 40030 Da SUBUNIT: Monomer. Probably homodimer formed by 2 molecules on adjacent cells. Interacts with LNX, MPDZ, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Binds adenovirus 12 fiber protein. Isoform 5 but not isoform 3 binds to coxsackievirus. SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. TISSUE SPECIFICITY: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitoulsy expressed while isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy. DOMAIN: SwissProt: P78310 The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML. & The PDZ-binding motif mediates interaction with MPDZ and BAIAP1. PTM: N-glycosylated (By similarity). & Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Molecular Weight	~46 kDa

Biological Information

Immunogen

Native human coxsackie virus-adenovirus receptor protein.

Clone

RmcB

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Mouse

Specificity

Recognizes CAR at MW ~46 kDa.

Isotype

IgG1

Species Reactivity

Human
Mouse
Rat

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NM_001338

Entrez Gene Summary

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.

Gene Symbol

CXADR
CAR
hCAR
HCVADR
HCAR

Purification Method

Protein G Purified

UniProt Number

P78310

UniProt Summary

FUNCTION: SwissProt: P78310 # Receptor for group B coxsackieviruses and subgroup C of adenoviruses (AD2 and AD5), susceptibility to infection is correlated to its level of expression and availability at the plasma membrane of cells.
SIZE: 365 amino acids; 40030 Da
SUBUNIT: Monomer. Probably homodimer formed by 2 molecules on adjacent cells. Interacts with LNX, MPDZ, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Binds adenovirus 12 fiber protein. Isoform 5 but not isoform 3 binds to coxsackievirus.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted.
TISSUE SPECIFICITY: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitoulsy expressed while isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
DOMAIN: SwissProt: P78310 The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML. & The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
PTM: N-glycosylated (By similarity). & Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Molecular Weight

~46 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on mouse small intestine lysates. Western Blot Analysis: 1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot on mouse small intestine lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	200 µg

Packaging Information

Material Size

200 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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