Anti-Kasio, Clone 6F

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货号：05-659

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
Ca, Ch, H, Ht, M, Mk, R	EMSA, IP, WB, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	05-659
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-Kaiso Antibody, clone 6F

References

Product Information
Format	Purified
Presentation	0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.

Applications
Application	Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.
Key Applications	Electrophoretic Mobility Shift Assay Immunoprecipitation Western Blotting Immunohistochemistry

Biological Information
Immunogen	Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso
Clone	clone 6F
Host	Mouse
Specificity	Kaiso
Isotype	IgG1
Species Reactivity	Canine Chicken Human Hamster Mouse Monkey Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_006777
Gene Symbol	ZBTB33 ZNF348 kaiso ZNF-kaiso WUGSC:H_DJ525N14.1 KAISO
Purification Method	Protein G Chromatography
UniProt Number	Q86T24
UniProt Summary	FUNCTION: SwissProt: Q86T24 # Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. SIZE: 672 amino acids; 74484 Da SUBUNIT: Self-associates. Interacts with CTNND2 (By similarity). Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with NCOR1. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Also cytoplasmic in cells grown at high densities. TISSUE SPECIFICITY: Expressed in vascular endothelium. SIMILARITY: SwissProt: Q86T24 ## Contains 1 BTB (POZ) domain. & Contains 3 C2H2-type zinc fingers.
Molecular Weight	97kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells. Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999). Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001). Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	2 years at -20°C

Packaging Information
Material Size	200 µg

Transport Information

Supplemental Information

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