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Rb X PRMT5 (C-TERM)

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货号：AB3349

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Rb Purified Polyclonal Antibody
Description
Catalogue Number AB3349
Replaces MABE551
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-PRMT5 Antibody, CT
References
Product Information
Format Purified
Control
  • POSITIVE CONTROL: HL60 cell lysates.
Presentation Purified immunoglobulin in PBS containing 0.09% sodium azide.
Applications
Application Anti-PRMT5 Antibody, C-terminus is a high quality Rabbit Polyclonal Antibody for the detection of PRMT5 & has been validated in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application Notes Western Blot: 1:100-1:500. Reacts with a band of approximately 70 kDa on whole HL60 cell lysate. Additional bands may be present depending on sample used.

ELISA (direct): 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide from C-terminus of human PRMT5.
Epitope C-terminus
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Reacts with PRMT5.
Species Reactivity
  • Human
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRMT5
  • SKB1
  • HRMT1L5
  • IBP72
  • SKB1Hs
  • JBP1
  • EC 2.1.1.125
  • EC 2.1.1.-
UniProt Number
UniProt Summary FUNCTION: SwissProt: O14744 # Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.
SIZE: 637 amino acids; 72684 Da
SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Disulfide bonds and non-covalent association mediate homooligomers formation.
SIMILARITY: SwissProt: O14744 ## Belongs to the protein arginine N-methyltransferase family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 400 µL
Transport Information
Supplemental Information
Specifications

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