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Anti-trimethyl Histone H3 (Lys4), Alexa Fluor?555 Conjugate

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货号：07-473-AF555

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M ICC Rb AlexaFluor®555 Polyclonal Antibody
Description
Catalogue Number 07-473-AF555
Description Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate
Alternate Names
  • H3K4me3
  • H3.3B, Lys4 trimethylated
  • H3 histone, family 3A, Lys4 trimethylated
  • H3 histone, family 3B, Lys4 trimethylated
  • Histone H3, Lys4 trimethylated
Background Information Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
Format AlexaFluor®555
Presentation Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Applications
Application This Anti-trimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3.
Key Applications
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected detected histone H3 Lys4 trimethylation in HeLa cells.
The unconjugated antibody (Cat. No. 07-473) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, and Western blotting applications.
Biological Information
Immunogen Synthetic peptide corresponding to residues surrounding and including trimethlyated Lys4 of histone H3.
Epitope Trimethylated Lys4 (H3K4me3).
Concentration Please refer to lot specific datasheet.
Host Rabbit
Specificity This antibody only detects histone H3 when trimethylated on Lys4.
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Human, Mouse. Expected to react with most other vertebrate species based on highly conserved sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FB
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FD
  • HIST1H3E
  • HIST1H3F
  • H3FF
  • HIST1H3G
  • HIST1H3H
  • H3FH
  • HIST1H3I
  • H3FI
  • HIST1H3J
  • H3FJ
  • H3FK
  • HIST3H3
  • H3FT
  • H3T
Modifications
  • Methylation
Purification Method Affinity purified.
UniProt Number
Molecular Weight ~16 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys4 trimethylation in HepG2 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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