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Rb X Ki-67

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货号：AB9260

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R IH(P), WB Rb Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB9260
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Ki-67 Antibody
Background Information Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.
References
Product Information
Format Affinity Purified
Control
  • Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.
Presentation Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.
Applications
Application Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application Notes Immunocytochemistry:
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).

Western Blot Analysis:
A previous lot of this antibody was used in western blot.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide from human Ki-67
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rabbit
Specificity Recognizes Ki-67
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Human and mouse. Expected to react with rat based on sequence homology.
Antibody Type Polyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MKI67
  • KIA
  • Ki-67
Purification Method ImmunoAffinity Purified
UniProt Number
UniProt Summary FUNCTION: SwissProt: P46013 # Thought to be required for maintaining cell proliferation.
SIZE: 3256 amino acids; 358694 Da
SUBUNIT: Binds through the FHA domain to MKI67IP.
SUBCELLULAR LOCATION: Nucleus. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
DEVELOPMENTAL STAGE: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
SIMILARITY: SwissProt: P46013 ## Contains 1 FHA domain.
Molecular Weight Predicted: 359 kDa. MW may be observed at ~345 or 395 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size 500 µL
Transport Information
Supplemental Information
Specifications

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