Anti-RNF2 (Ring1b) (rabbit polyclonal)

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R, B, Ch, Ca	WB	Rb	Affinity Purified	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R, B, Ch, Ca

Affinity Purified

Polyclonal Antibody

Description
Catalogue Number	09-723
Description	Anti-RNF2 (Ring1b) Antibody
Alternate Names	DinG protein HIP2-interacting protein 3 Huntingtin-interacting protein 2-interacting protein 3 RING finger protein 1B RING finger protein BAP-1 ring finger protein 2
Background Information	RNF2 (ring finger 2), also known as Ring1b, is one of the polycomb groups of proteins, which are important for proper development and stem cell renewal. These proteins form complexes that are essential for the transcriptional repression of genes involved in cell proliferation. This is done through post-translational modification and binding to histones. RNF2 is a critical component of that process. Changes in the expression of these proteins have been linked to cancer. RNF2 has been shown to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme. Studies have also shown that RNF2 is able to suppress the activity of transcription factor CP2.

Description

Catalogue Number

09-723

Description

Anti-RNF2 (Ring1b) Antibody

Alternate Names

DinG protein
HIP2-interacting protein 3
Huntingtin-interacting protein 2-interacting protein 3
RING finger protein 1B
RING finger protein BAP-1
ring finger protein 2

Background Information

RNF2 (ring finger 2), also known as Ring1b, is one of the polycomb groups of proteins, which are important for proper development and stem cell renewal. These proteins form complexes that are essential for the transcriptional repression of genes involved in cell proliferation. This is done through post-translational modification and binding to histones. RNF2 is a critical component of that process. Changes in the expression of these proteins have been linked to cancer. RNF2 has been shown to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme. Studies have also shown that RNF2 is able to suppress the activity of transcription factor CP2.

Product Information
Format	Affinity Purified
Control	NIH/3T3 cell lysate
Presentation	Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Product Information

Format

Affinity Purified

Control

NIH/3T3 cell lysate

Presentation

Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Applications
Application	Use Anti-RNF2 (Ring1b) Antibody (rabbit polyclonal antibody) validated in WB to detect RNF2 (Ring1b) also known as DinG protein ,Huntingtin-interacting protein 2-interacting protein 3, RING finger protein BAP-1 .
Key Applications	Western Blotting
Application Notes	Western Blot Analysis: 1 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of human brain tissue lysate. Western Blot (SNAP ID) Analysis: 1.5 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.

Applications

Application

Use Anti-RNF2 (Ring1b) Antibody (rabbit polyclonal antibody) validated in WB to detect RNF2 (Ring1b) also known as DinG protein ,Huntingtin-interacting protein 2-interacting protein 3, RING finger protein BAP-1 .

Key Applications

Western Blotting

Application Notes

Western Blot Analysis: 1 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of human brain tissue lysate.
Western Blot (SNAP ID) Analysis:
1.5 µg/mL from a previous lot detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human RNF2 (Ring1b).
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This antibody recognizes RNF2 (Ring1b).
Species Reactivity	Human Mouse Rat Bovine Chicken Canine
Species Reactivity Note	Demonstrated to react with human, mouse, and rat. Predicted to react with chicken, canine (dog), and bovine (cow) based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_009143.1
Entrez Gene Summary	Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
Gene Symbol	BAP-1 BAP1 DING EC 6.3.2.- HIPI3 OTTHUMP00000033405 OTTHUMP00000060668 RING1B RING1b RING2
Purification Method	Affinity Purfied
UniProt Number	Q99496
UniProt Summary	FUNCTION: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 and BMI1/PCGF4 may rather act as a modulator of RNF2/RING2 activity. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT STRUCTURE: Component of some chromatin-associated Polycomb complex (PcG). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. SUBCELLULAR LOCATION: Nucleus By similarity. Note: Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells By similarity. SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger. SEQUENCE CAUTIION: The sequence CAI17849.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI17850.1 differs from that shown. Reason: Erroneous gene model prediction.
Molecular Weight	~45 kDa

Biological Information

Immunogen

KLH-conjugated linear peptide corresponding to human RNF2 (Ring1b).

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Rabbit

Specificity

This antibody recognizes RNF2 (Ring1b).

Species Reactivity

Human
Mouse
Rat
Bovine
Chicken
Canine

Species Reactivity Note

Demonstrated to react with human, mouse, and rat. Predicted to react with chicken, canine (dog), and bovine (cow) based on 100% sequence homology.

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NP_009143.1

Entrez Gene Summary

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

Gene Symbol

BAP-1
BAP1
DING
EC 6.3.2.-
HIPI3
OTTHUMP00000033405
OTTHUMP00000060668
RING1B
RING1b
RING2

Purification Method

Affinity Purfied

UniProt Number

Q99496

UniProt Summary

FUNCTION: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 and BMI1/PCGF4 may rather act as a modulator of RNF2/RING2 activity.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT STRUCTURE: Component of some chromatin-associated Polycomb complex (PcG). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of chromatin-associated class II PcG repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. SUBCELLULAR LOCATION: Nucleus By similarity. Note: Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells By similarity.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
SEQUENCE CAUTIION: The sequence CAI17849.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAI17850.1 differs from that shown. Reason: Erroneous gene model prediction.

Molecular Weight

~45 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in NIH/3T3 cell lysate. Western Blot Analysis: 0.5-1 µg/mL of this antibody detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: 0.5-1 µg/mL of this antibody detected RNF2 (Ring1b) in 10 µg of NIH/3T3 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable at 2-8°C for 1 year from date of recepit.

Storage and Shipping Information

Storage Conditions

Stable at 2-8°C for 1 year from date of recepit.

Packaging Information
Material Size	100 µg

Packaging Information

Material Size

100 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Anti-RNF2 (Ring1b) (rabbit polyclonal)

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